ArticlePloS one2013
The protective effect of intrasplenic transplantation of Ad-IL-18BP/IL-4 gene-modified fetal hepatocytes on ConA-induced hepatitis in mice.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Article
- 3D hESC exosomes enriched with miR-6766-3p ameliorates liver fibrosis by attenuating activated stellate cells through targeting the TGFβRII-SMADS pathway.Journal of nanobiotechnology · 2021Article
- Functional heterogeneity of CD4Seminars in immunopathology · 2021Review
- Inherited IL-18BP deficiency in human fulminant viral hepatitis.The Journal of experimental medicine · 2019Article
- The Protective Effect of Sheep Placental Extract on Concanavalin A-induced Liver Injury in Mice.Molecules (Basel, Switzerland) · 2018Article
- Serum Levels of IL-33 and Correlation with IL-4, IL-17A, and Hypergammaglobulinemia in Patients with Autoimmune Hepatitis.Mediators of inflammation · 2018Article
- Anti-CD200 attenuates concanavalin A induced hepatitis via modulating the imbalance of CD4American journal of translational research · 2018Article
- Methane alleviates carbon tetrachloride induced liver injury in mice: anti-inflammatory action demonstrated by increased PI3K/Akt/GSK-3β-mediated IL-10 expression.Journal of molecular histology · 2017Article
- Expression and roles of TIPE2 in autoimmune hepatitis.Experimental and therapeutic medicine · 2017Article
- Activated farnesoid X receptor attenuates apoptosis and liver injury in autoimmune hepatitis.Molecular medicine reports · 2015Article
- Paeoniflorin exerts a nephroprotective effect on concanavalin A-induced damage through inhibition of macrophage infiltration.Diagnostic pathology · 2015Article
- Ghrelin reduces liver impairment in a model of concanavalin A-induced acute hepatitis in mice.Drug design, development and therapy · 2015Article
- Hydrogen sulfide, a potential novel drug, attenuates concanavalin A-induced hepatitis.Drug design, development and therapy · 2014Article
- Salidroside attenuates concanavalin A-induced hepatitis via modulating cytokines secretion and lymphocyte migration in mice.Mediators of inflammation · 2014Article
- Successful auto-implantation of hepatic cells in lung tissue: An animal study.Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · 2013Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundConcanavalin A (ConA)-induced hepatitis is an experimental murine model mirroring the pathology of human autoimmune hepatitis.
aimTo investigate the effects of intrasplenically transplanted fetal hepatocytes (BNL.CL2) transfected with recombinant adenovirus vector expressing the IL-18 binding protein (IL-18BP) and IL-4 fusion protein on ConA-induced hepatitis in mice.
methodsAd-IL-18BP/IL-4 was used to infect BNL.CL2 cells. IL-4 and IL-18BP fusion protein expression were detected by ELISA and Western blotting. BNL.CL2 cells infected with Ad-IL-18BP/IL-4 were intrasplenically transplanted into mice. After 10 days, mice were injected with ConA (15 mg/kg), and sacrificed 18 hours later. Liver injury was assessed by serum transaminase and liver histology. TNF-α, IL-18, IL-4, IL-10, IL-12p70 and monocyte-chemoattracting protein (MCP)-1 levels in serum and liver homogenates were detected by ELISA. Signaling molecules in liver homogenates were analyzed by Western blotting.
resultsAd-IL-18BP/IL-4 effectively expressed the IL-18BP/IL-4 fusion protein for more than 14 days in BNL.CL12 cells. Treatment of mice with Ad-IL-18BP/IL-4-BNL.CL2 before ConA injection significantly reduced the elevated plasma levels of transaminases compared with ConA control groups. TNF-α, IL-18, IL-12p70 and MCP-1 levels in serum and liver homogenates from mice transplanted with Ad-IL-18BP/IL-4-BNL.CL2 were lower and IL-4 and IL-10 levels were higher than control groups. Phosphorylation levels of NF-κB p65, AKT, p38 and JNK1/2 in liver homogenates were markedly suppressed by Ad-IL-18BP/IL-4.
conclusionsAd-IL-18BP/IL-4 was effectively transfected into mouse BNL.CL2 cells. Intrasplenic transplantation of Ad-IL-18BP/IL-4-BNL.CL12 cells alleviated the severity of inflammation in ConA-induced experimental hepatitis and provides a useful basis for the targeted gene therapy of liver disease.
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