ArticlePloS one2013
The mitochondria-mediate apoptosis of Lepidopteran cells induced by azadirachtin.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 81 citations in OpenAlex.
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- The Survival and Physiological Response ofInsects · 2024Article
- The Changes in Mitochondrial Morphology and Physiology Accompanying Apoptosis inInternational journal of molecular sciences · 2023Article
- Insect Cell-Based Models: Cell Line Establishment and Application in Insecticide Screening and Toxicology Research.Insects · 2023Review
- Conceptual framework for the insect metamorphosis from larvae to pupae by transcriptomic profiling, a case study of Helicoverpa armigera (Lepidoptera: Noctuidae).BMC genomics · 2022Article
- Oxidative Stress Contributes to Cytoskeletal Protein Degradation ofFoods (Basel, Switzerland) · 2022Article
- Exploring the role of3 Biotech · 2021Review
- A Novel Insecticidal Molecule Extracted fromInsects · 2020Article
- Acetylcholinesterase promotes apoptosis in insect neurons.Apoptosis : an international journal on programmed cell death · 2020Article
- Growth Performance and Enzymatic Response of the Grasshopper, Calliptamus abbreviatus (Orthoptera: Acrididae), to Six Plant-Derived Compounds.Journal of insect science (Online) · 2020Article
- Stability of selected reference genes in Sf9 cells treated with extrinsic apoptotic agents.Scientific reports · 2019Article
- DnaJ homolog subfamily A member1 (DnaJ1) is a newly discovered anti-apoptotic protein regulated by azadirachtin in Sf9 cells.BMC genomics · 2018Article
- Cytotoxic and Apoptotic Activity of the Novel Harmine Derivative ZC-14 in Sf9 Cells.International journal of molecular sciences · 2018Article
- Azadirachtin Affects the Growth ofFrontiers in physiology · 2018Article
- Transcriptome analysis of Spodoptera frugiperda Sf9 cells reveals putative apoptosis-related genes and a preliminary apoptosis mechanism induced by azadirachtin.Scientific reports · 2017Article
- Production of biopesticide azadirachtin using plant cell and hairy root cultures.Engineering in life sciences · 2017Review
- Isoliquiritigenin induces apoptosis of human bladder cancer T24 cells via a cyclin-dependent kinase-independent mechanism.Oncology letters · 2017Article
- Azadirachtin induced apoptosis in the prothoracic gland inInternational journal of biological sciences · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitochondria have been shown to play an important role in apoptosis using mammalian cell lines. However, this seems not to be the case in Drosophila, an insect model organism; thus more in-depth studies of insect cell apoptosis are necessary. In the present study, mitochondrial involvement during azadirachtin- and camptothecin-induced apoptosis in Spodoptera frugiperda Sf9 cells (isolated from Spodoptera frugiperda pupal ovarian tissue) was investigated. The results showed that both azadirachtin and camptothecin could induce apoptosis in Sf9 cells. Reactive oxygen species (ROS) generation, activation of mitochondrial permeability transition pores (MPTPs) and loss of mitochondrial membrane potential (MMP) were observed very early during apoptosis and were followed subsequently by the release of cytochrome-c from the mitochondria. Furthermore, the results also revealed that the opening of MPTPs and the loss of MMP induced by azadirachtin could be significantly inhibited by the permeability transition pore (PTP) inhibitor cyclosporin A (CsA), which was used to identify the key role of mitochondria in the apoptosis of Sf9 cells. However, in camptothecin-treated Sf9 cells, CsA could not suppress the opening of MPTPs and the loss of MMP when apoptosis was induced. The data from caspase-3 and caspase-9 activity assays and detection of apoptosis by morphological observation and flow cytometry also uncovered the different effect of CsA on the two botanical apoptosis inducers. Although different mechanisms of apoptosis induction exist, our study revealed that mitochondria play a crucial role in insect cell line apoptosis.
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