ArticlePloS one2013
Consuming transgenic goats' milk containing the antimicrobial protein lysozyme helps resolve diarrhea in young pigs.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 58 citations in OpenAlex.
- Immunomodulatory Role of Milk on Gut Microbiota: Implications for Enhancing Oral Vaccine Efficacy.Probiotics and antimicrobial proteins · 2026Review
- Preserving Mediterranean Donkeys: A Study on Milk Production and Nutritional Benefits.Animals : an open access journal from MDPI · 2024Article
- Article
- Milk Bioactive Compounds and Gut Microbiota Modulation: The Role of Whey Proteins and Milk Oligosaccharides.Foods (Basel, Switzerland) · 2024Review
- Invited review: strategic adoption of antibiotic-free pork production: the importance of a holistic approach.Translational animal science · 2022Review
- Towards progressive regulatory approaches for agricultural applications of animal biotechnology.Transgenic research · 2022Review
- Responses of Vaginal Microbiota to Dietary Supplementation with Lysozyme and its Relationship with Rectal Microbiota and Sow Performance from Late Gestation to Early Lactation.Animals : an open access journal from MDPI · 2021Article
- Maternal Nutrition During Late Gestation and Lactation: Association With Immunity and the Inflammatory Response in the Offspring.Frontiers in immunology · 2021Review
- Recent Insights Into the Prognostic and Therapeutic Applications of Lysozymes.Frontiers in pharmacology · 2021Review
- Fecal bacteria and metabolite responses to dietary lysozyme in a sow model from late gestation until lactation.Scientific reports · 2020Article
- Functional evaluation of a monotreme-specific antimicrobial protein, EchAMP, against experimentally induced mastitis in transgenic mice.Transgenic research · 2019Article
- Dietary Lysozyme Alters Sow's Gut Microbiota, Serum Immunity and Milk Metabolite Profile.Frontiers in microbiology · 2019Article
- Effects of dietary supplementation with lysozyme during late gestation and lactation stage on the performance of sows and their offspring.Journal of animal science · 2018Article
- Unprotonated Short-Chain Alkylamines Inhibit Staphylolytic Activity of Lysostaphin in a Wall Teichoic Acid-Dependent Manner.Applied and environmental microbiology · 2018Article
- Lysozyme improves gut performance and protects against enterotoxigenic Escherichia coli infection in neonatal piglets.Veterinary research · 2018Article
- EMA and EFSA Joint Scientific Opinion on measures to reduce the need to use antimicrobial agents in animal husbandry in the European Union, and the resulting impacts on food safety (RONAFA).EFSA journal. European Food Safety Authority · 2017Article
- Large-scale production of functional human lysozyme from marker-free transgenic cloned cows.Scientific reports · 2016Article
- Salivary microbiota reflects changes in gut microbiota in cirrhosis with hepatic encephalopathy.Hepatology (Baltimore, Md.) · 2015Article
- Production of human lactoferrin and lysozyme in the milk of transgenic dairy animals: past, present, and future.Transgenic research · 2015Review
- Production of transgenic-cloned pigs expressing large quantities of recombinant human lysozyme in milk.PloS one · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Childhood diarrhea is a significant problem in many developing countries and E. coli is a main causative agent of diarrhea in young children. Lysozyme is an antimicrobial protein highly expressed in human milk, but not ruminant milk, and is thought to help protect breastfeeding children against diarrheal diseases. We hypothesized that consumption of milk from transgenic goats which produce human lysozyme (hLZ-milk) in their milk would accelerate recovery from bacterial-induced diarrhea. Young pigs were used as a model for children and infected with enterotoxigenic E. coli. Once clinical signs of diarrhea developed, pigs were fed hLZ-milk or non-transgenic control goat milk three times a day for two days. Clinical observations and complete blood counts (CBC) were performed. Animals were euthanized and samples collected to assess differences in histology, cytokine expression and bacterial translocation into the mesenteric lymph node. Pigs consuming hLZ-milk recovered from clinical signs of infection faster than pigs consuming control milk, with significantly improved fecal consistency (p = 0.0190) and activity level (p = 0.0350). The CBC analysis showed circulating monocytes (p = 0.0413), neutrophils (p = 0.0219), and lymphocytes (p = 0.0222) returned faster to pre-infection proportions in hLZ-milk fed pigs, while control-fed pigs had significantly higher hematocrit (p = 0.027), indicating continuing dehydration. In the ileum, pigs fed hLZ-milk had significantly lower expression of pro-inflammatory cytokine IL-8 (p = 0.0271), longer intestinal villi (p<0.0001), deeper crypts (p = 0.0053), and a thinner lamina propria (p = 0.0004). These data demonstrate that consumption of hLZ-milk helped pigs recover from infection faster, making hLZ-milk an effective treatment of E. coli-induced diarrhea.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.