ArticlePloS one2013
Membrane potential measurements of isolated neurons using a voltage-sensitive dye.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
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- Quantification of kappa opioid receptor ligand potency, efficacy and desensitization using a real-time membrane potential assay.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2021Article
- Role of 5-HT1A and 5-HT3 receptors in serotonergic activation of sensory neurons in relation to itch and pain behavior in the rat.Journal of neuroscience research · 2020Article
- All-Optical Detection of Neuronal Membrane Depolarization in Live Cells Using Colloidal Quantum Dots.Nano letters · 2019Article
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- The Applications of Lattice Light-sheet Microscopy for Functional Volumetric Imaging of Hippocampal Neurons in a Three-Dimensional Culture System.Micromachines · 2019Article
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- Rapid Throughput Analysis of GABAMolecular pharmacology · 2017Article
- Photoacoustic imaging of voltage responses beyond the optical diffusion limit.Scientific reports · 2017Article
- Association between chronic stress-induced structural abnormalities in Ranvier nodes and reduced oligodendrocyte activity in major depression.Scientific reports · 2016Article
- Oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles.International journal of nanomedicine · 2016Article
- Low-dose ionizing radiation induces mitochondrial fusion and increases expression of mitochondrial complexes I and III in hippocampal neurons.Oncotarget · 2015Article
- Optical Tools to Investigate Cellular Activity in the Intestinal Wall.Journal of neurogastroenterology and motility · 2015Article
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Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to monitor changes in membrane potential is a useful tool for studying neuronal function, but there are only limited options available at present. Here, we have investigated the potential of a commercially available FLIPR membrane potential (FMP) dye, developed originally for high throughput screening using a plate reader, for imaging the membrane potential of cultured cells using an epifluorescence-based single cell imaging system. We found that the properties of the FMP dye make it highly suitable for such imaging since 1) its fluorescence displayed a high signal-to-noise ratio, 2) robust signals meant only minimal exposure times of around 5 ms were necessary, and 3) bidirectional changes in fluorescence were detectable resulting from hyper- or depolarising conditions, reaching equilibrium with a time constant of 4-8 s. Measurements were possible independently of whether membrane potential changes were induced by voltage clamping, or manipulating the ionic distribution of either Na(+) or K(+). Since FMP behaves as a charged molecule which accumulates in the cytosol, equations based on the Boltzmann distribution were developed determining that the apparent charge of FMP which represents a measure of the voltage sensitivity of the dye, is between -0.62 and -0.72. Finally, we demonstrated that FMP is suitable for use in a variety of neuronal cell types and detects membrane potential changes arising from spontaneous firing of action potentials and through stimulation with a variety of excitatory and inhibitory neurotransmitters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.