ArticlePloS one2013
Isolation of rare tumor cells from blood cells with buoyant immuno-microbubbles.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Advances on Liquid Biopsy Analysis for Glioma Diagnosis.Biomedicines · 2023Review
- Efficacy optimization of low frequency microbubble-mediated sonoporation as a drug delivery platform to cancer cells.International journal of pharmaceutics: X · 2022Article
- Laboratory-Scale Production of Sterile Targeted Microbubbles.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- Lipid Microbubble-Conjugated Anti-CD3 and Anti-CD28 Antibodies (Microbubble-Based Human T Cell Activator) Offer Superior Long-Term Expansion of Human Naive T Cells In Vitro.ImmunoHorizons · 2020Article
- Nano Meets Micro-Translational Nanotechnology in Medicine: Nano-Based Applications for Early Tumor Detection and Therapy.Nanomaterials (Basel, Switzerland) · 2020Review
- Microfluidics for studying metastatic patterns of lung cancer.Journal of nanobiotechnology · 2019Review
- Enhanced capture and release of circulating tumor cells using hollow glass microspheres with a nanostructured surface.Nanoscale · 2018Article
- A tumor-myeloid cell axis, mediated via the cytokines IL-1α and TSLP, promotes the progression of breast cancer.Nature immunology · 2018Article
- Isolation of Breast cancer CTCs with multitargeted buoyant immunomicrobubbles.Colloids and surfaces. B, Biointerfaces · 2018Article
- MicroBubble activated acoustic cell sorting.Biomedical microdevices · 2017Article
- Novel method for the formation of monodisperse superheated perfluorocarbon nanodroplets as activatable ultrasound contrast agents.RSC advances · 2017Article
- A microfluidic platform reveals differential response of regulatory T cells to micropatterned costimulation arrays.Integrative biology : quantitative biosciences from nano to macro · 2015Article
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- Article
- The behavior of lipid debris left on cell surfaces from microbubble based ultrasound molecular imaging.Ultrasonics · 2014Article
- Article
- Binding and isolation of tumor cells in biological media with perfluorocarbon microbubbles.Methods (San Diego, Calif.) · 2013Article
- In vivo ultrasound visualization of non-occlusive blood clots with thrombin-sensitive contrast agents.Biomaterials · 2013Article
- Stimulus-responsive ultrasound contrast agents for clinical imaging: motivations, demonstrations, and future directions.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnologyReview
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Circulating tumor cells (CTCs) are exfoliated at various stages of cancer, and could provide invaluable information for the diagnosis and prognosis of cancers. There is an urgent need for the development of cost-efficient and scalable technologies for rare CTC enrichment from blood. Here we report a novel method for isolation of rare tumor cells from excess of blood cells using gas-filled buoyant immuno-microbubbles (MBs). MBs were prepared by emulsification of perfluorocarbon gas in phospholipids and decorated with anti-epithelial cell adhesion molecule (EpCAM) antibody. EpCAM-targeted MBs efficiently (85%) and rapidly (within 15 minutes) bound to various epithelial tumor cells suspended in cell medium. EpCAM-targeted MBs efficiently (88%) isolated frequent tumor cells that were spiked at 100,000 cells/ml into plasma-depleted blood. Anti-EpCAM MBs efficiently (>77%) isolated rare mouse breast 4T1, human prostate PC-3 and pancreatic cancer BxPC-3 cells spiked into 1, 3 and 7 ml (respectively) of plasma-depleted blood. Using EpCAM targeted MBs CTCs from metastatic cancer patients were isolated, suggesting that this technique could be developed into a valuable clinical tool for isolation, enumeration and analysis of rare cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.