ArticleJournal of clinical microbiology2013
Direct bacterial identification in positive blood cultures by use of two commercial matrix-assisted laser desorption ionization-time of flight mass spectrometry systems.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
71 citing papers in PubMed, 1 synthesis or guideline pooled it, 165 citations in OpenAlex.
- A systematic review of matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry compared to routine microbiological methods for the time taken to identify microbial organisms from positive blood cultures.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Pooled it
- [Requirements for the rational use of antibiotics based on antibiotic stewardship].Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz · 2026Review
- Direct identification and susceptibility testing of positive blood cultures: a large-scale evaluation of an ammonium chloride-based in-house workflow.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- First Detection of Lactococcus formosensis subsp. formosensis in Rainbow Trout (Oncorhynchus mykiss) in Europe.Journal of fish diseases · 2026Article
- Comparative evaluation of two MALDI-TOF MS systems for microbial identification: accuracy and workflow efficiency in a clinical microbiology laboratory.Microbiology spectrum · 2026Article
- Rapid identification and antimicrobial susceptibility testing of pathogenic microorganisms from positive blood cultures.PloS one · 2026Article
- Plug-and-Play Centrifuge-Only Device for Rapid Sepsis Diagnosis.Advanced healthcare materials · 2025Article
- Accuracy of two Sepsityper MALDI-TOF MS methods for bacterial identification in bloodstream infections in dogs, foals, and calves using Bayesian latent class model.The veterinary quarterly · 2025Article
- Rapid colorimetric antimicrobial susceptibilities direct from positive blood culture for Gram-negative bacteria.Microbiology spectrum · 2025Article
- Antimicrobial susceptibility and genomic characterization ofMicrobiology spectrum · 2025Article
- Direct identification and antimicrobial susceptibility testing of microorganisms from positive blood culture bottles using a membrane filtration method.BMC microbiology · 2025Article
- Isolation and identification of bacteria from blood within 12 h using standard laboratory equipment.Scientific reports · 2025Article
- Spectral Precision: Recent Advances in Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Pathogen Detection and Resistance Profiling.Microorganisms · 2025Review
- Utility of digital images captured after 4 h of incubation on a microbiology laboratory automation system in guiding the work-up of subcultures from positive blood cultures.Journal of clinical microbiology · 2025Article
- An investigation of scattered light integrating collector technology for rapid blood culture sensitivity testing.Journal of medical microbiology · 2024Article
- Genomic investigation ofMicrobiology spectrum · 2024Article
- Potassium affects the association between dietary intake of vitamin C and NAFLD among adults in the United States.PloS one · 2024Article
- Evaluation of Two Tests for the Rapid Detection of CTX-M Producers Directly in Urine Samples.Antibiotics (Basel, Switzerland) · 2023Article
- Comparison of the New VITEK MS PRIME System with the Matrix-Assisted Laser Desorption Ionization Biotyper Microflex LT for the Identification of Microorganisms.Annals of laboratory medicine · 2023Article
- Draft genome sequence of biofilm-formingMicrobiology resource announcements · 2023Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the identification of bacteria and fungi was recently introduced in microbiology laboratories. This technology could greatly improve the clinical management of patients and guidance for chemotherapy. In this study, we used a commercial MALDI Sepsityper extraction method to evaluate the performance of two commercial MALDI-TOF MS systems, the Vitek MS IVD (bioMérieux) and the Microflex LT Biotyper (Bruker Daltonics) for direct bacterial identification in positive blood cultures. In 181 monomicrobial cultures, both systems generated genus to species level identifications for >90% of the specimens (Biotyper, 177/181 [97.8%]; Vitek MS IVD, 167/181 [92.3%]). Overall, the Biotyper system generated significantly more accurate identifications than the Vitek MS IVD system (P = 0.016; 177 versus 167 out of 181 specimens). The Biotyper system identified the minority species among polymicrobial blood cultures. We also compared the performance of an in-house extraction method with that of the Sepsityper on both MALDI-TOF MS systems. The in-house method generated more correct identifications at the genus level than the Sepsityper (96.7% versus 93.5%) on the Biotyper system, whereas the two methods exhibited the same performance level (88.0% versus 88.0%) on the Vitek MS IVD system. Our study confirmed the practical advantages of MALDI-TOF MS, and our in-house extraction method reduced the reagent cost to $1 per specimen, with a shorter turnaround time of 3 h, which is highly cost-effective for a diagnostic microbiology service.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.