ArticleBioResearch open access2013
A method to evaluate the efficiency of transfection reagents in an adherent zebrafish cell line.
Article in BioResearch open access, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- High-efficiency transfection of Acanthamoeba castellanii using a cationic polymer.Cell reports methods · 2026Article
- A Simple and Versatile Method for Ex Vivo Monitoring of Goat Vaginal Mucosa Transduction by Viral Vector Vaccines.Vaccines · 2024Article
- A non-viral DNA delivery system consisting of multifunctional chimeric peptide fused with zinc-finger protein.iScience · 2024Article
- Optimization of specific RNA knockdown in mammalian cells with CRISPR-Cas13.Methods (San Diego, Calif.) · 2022Article
- Development and characterization of a new gill cell line from the striped catfish, Pangasianodon hypophthalmus (Sauvage, 1878).Fish physiology and biochemistry · 2022Article
- A Stable CHO K1 Cell Line for Producing Recombinant Monoclonal Antibody Against TNF-α.Molecular biotechnology · 2021Article
- Article
- Lentivirus Manufacturing Process for Primary T-Cell Biofactory Production.Advanced biosystems · 2020Article
- Article
- Gene delivery into Siberian sturgeon cell lines by commercial transfection reagents.In vitro cellular & developmental biology. Animal · 2019Article
- Preparation and Characterization of PLA-PEG-PLA/PEI/DNA Nanoparticles for Improvement of Transfection Efficiency and Controlled Release of DNA in Gene Delivery Systems.Iranian journal of pharmaceutical research : IJPR · 2019Article
- Efficient Nonviral Transfection of Human Bone Marrow Mesenchymal Stromal Cells Shown Using Placental Growth Factor Overexpression.Stem cells international · 2018Article
- An improved method for increasing the efficiency of gene transfection and transduction.International journal of physiology, pathophysiology and pharmacology · 2018Article
- A novel rapid and reproducible flow cytometric method for optimization of transfection efficiency in cells.PloS one · 2017Article
- Gene therapy based on interleukin-12 loaded chitosan nanoparticles in a mouse model of fibrosarcoma.Iranian journal of basic medical sciences · 2016Article
- Kidney Injury Molecule-1 Enhances Endocytosis of Albumin in Renal Proximal Tubular Cells.Journal of cellular physiology · 2016Article
- Cadmium Protection Strategies--A Hidden Trade-Off?International journal of molecular sciences · 2016Review
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a simple and robust method to evaluate the transfection efficiency of commercially available transfection reagents intended to be established for use in nonmammalian cell lines. To illustrate the method, we compare the ability of four different reagents to transfect the embryonic zebrafish cell line Z3. Z3 cells were seeded in a 96-well plate and simultaneously transfected in several variations by using minimum volumes of transfection reagent and a vector DNA encoding an amplified version of green fluorescent protein (GFP). After 24 and 48 h, transfection efficiency was determined by a dual fluorescence plate reader measurement of GFP and Hoechst 33342 fluorescence, an indicator of cell density. Of the four different reagents tested, certain variations of JetPrime(™) reagent and X-tremeGene(™) HP reagent produced the highest fluorescence signal per cell after 24- and 48-h incubation, respectively. The simultaneous multivariate setup enables comparing different reagent/DNA combinations at different time points well, independent of cell growth variability or seeding density.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.