ArticleBioResearch open access2012
A standardized rat model of volumetric muscle loss injury for the development of tissue engineering therapies.
Article in BioResearch open access, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 154 citations in OpenAlex.
- Integrating and Orchestrating Multiple Components of Skeletal Muscle Injury and Regeneration.American journal of physiology. Cell physiology · 2026Article
- Anisotropic mechanotransductive tissue constructsBioactive materials · 2026Article
- Application of Fibrin-Laminin Hydrogel Concurrent with Electrically Stimulated Eccentric Training Hinders Recovery in Volumetric Muscle Loss.Journal of functional biomaterials · 2026Article
- Conductive MXene/adECM hydrogel promotes skeletal muscle regeneration and innervation through CaJournal of nanobiotechnology · 2026Article
- Catalyst-Free Collagen Filament Crosslinking for Engineering Anisotropic and Mechanically Robust Tissue Scaffolds.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Bioengineered Constructs as a Tissue Engineering-Based Therapy for Volumetric Muscle Loss.Tissue engineering. Part B, Reviews · 2026Review
- Postnatal Maxillofacial "Developing" Decellularized Extracellular Matrix Orchestrates Hierarchical Cross-Organ Regeneration via Macrophage Integrin αvβ5-Mediated Efferocytosis-Driven Developmental Recapitulation.Research (Washington, D.C.) · 2026Article
- Bioinspired hydrogel-based regenerative engineering strategies for the structural and functional regeneration of skeletal muscles following volumetric muscle loss.Regenerative biomaterials · 2026Review
- Critical-Size Muscle Defect Regeneration Using an Injectable Cell-Laden Nanofibrous Matrix: An Ex Vivo Mouse Hindlimb Organ Culture Study.International journal of molecular sciences · 2025Article
- Retrospective transcriptomic analysis indicates temporal dysregulation of mitochondrial genes and metabolic pathways after volumetric muscle loss injury.Physiological reports · 2025Article
- Evaluation of biological sex on endstage pathobiology and regenerative treatment of volumetric muscle loss.Scientific reports · 2025Article
- Freeze-Dried Porous Collagen Scaffolds for the Repair of Volumetric Muscle Loss Injuries.ACS biomaterials science & engineering · 2025Article
- Cell-scale porosity minimizes foreign body reaction and promotes innervated myofiber formation after volumetric muscle loss.NPJ Regenerative medicine · 2025Article
- Biomaterial-Based Regenerative Strategies for Volumetric Muscle Loss: Challenges and Solutions.Advances in wound care · 2025Review
- A mouse model of volumetric muscle loss and therapeutic scaffold implantation.Nature protocols · 2025Review
- Temporal dynamics of gene and protein signatures following volumetric muscle loss.Frontiers in cell and developmental biology · 2025Article
- Freeze-dried porous collagen scaffolds for the repair of volumetric muscle loss injuries.bioRxiv : the preprint server for biology · 2024Article
- Enhancing volumetric muscle loss (VML) recovery in a rat model using super durable hydrogels derived from bacteria.Bioactive materials · 2024Article
- A critical size volumetric muscle loss model in mouse masseter with impaired mastication on nutrition.Cell proliferation · 2024Article
- Fabrication of fully aligned self-assembled cell-laden collagen filaments for tissue engineeringBioactive materials · 2024Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Soft tissue injuries involving volumetric muscle loss (VML) are defined as the traumatic or surgical loss of skeletal muscle with resultant functional impairment and represent a challenging clinical problem for both military and civilian medicine. In response, a variety of tissue engineering and regenerative medicine treatments are under preclinical development. A wide variety of animal models are being used, all with critical limitations. The objective of this study was to develop a model of VML that was reproducible and technically uncomplicated to provide a standardized platform for the development of tissue engineering and regenerative medicine solutions to VML repair. A rat model of VML involving excision of ∼20% of the muscle's mass from the superficial portion of the middle third of the tibialis anterior (TA) muscle was developed and was functionally characterized. The contralateral TA muscle served as the uninjured control. Additionally, uninjured age-matched control rats were also tested to determine the effect of VML on the contralateral limb. TA muscles were assessed at 2 and 4 months postinjury. VML muscles weighed 22.7% and 19.5% less than contralateral muscles at 2 and 4 months postinjury, respectively. These differences were accompanied by a reduction in peak isometric tetanic force (Po) of 28.4% and 32.5% at 2 and 4 months. Importantly, Po corrected for differences in body weight and muscle wet weights were similar between contralateral and age-matched control muscles, indicating that VML did not have a significant impact on the contralateral limb. Lastly, repair of the injury with a biological scaffold resulted in rapid vascularization and integration with the wound. The technical simplicity, reliability, and clinical relevance of the VML model developed in this study make it ideal as a standard model for the development of tissue engineering solutions for VML.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.