ArticleMolecular biology of the cell2013
Xenopus cytoplasmic linker-associated protein 1 (XCLASP1) promotes axon elongation and advance of pioneer microtubules.
Article in Molecular biology of the cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Decoupling actin assembly from microtubule disassembly by TBC1D3C-mediated direct GEF-H1 activation.Life science alliance · 2025Article
- The TOG5 domain of CKAP5 is required to interact with F-actin and promote microtubule advancement in neurons.Molecular biology of the cell · 2024Article
- CKAP5 enables formation of persistent actin bundles templated by dynamically instable microtubules.Current biology : CB · 2024Article
- KIF2A deficiency causes early-onset neurodegeneration.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- TAOK2 rescues autism-linked developmental deficits in a 16p11.2 microdeletion mouse model.Molecular psychiatry · 2022Article
- With the Permission of Microtubules: An Updated Overview on Microtubule Function During Axon Pathfinding.Frontiers in molecular neuroscience · 2021Review
- CLASPs at a glance.Journal of cell science · 2020Review
- Dynein-mediated microtubule translocation powering neurite outgrowth in chick andJournal of cell science · 2020Article
- Profilin 1 delivery tunes cytoskeletal dynamics toward CNS axon regeneration.The Journal of clinical investigation · 2020Article
- Microtubules: Evolving roles and critical cellular interactions.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- XMAP215 promotes microtubule-F-actin interactions to regulate growth cone microtubules during axon guidance inJournal of cell science · 2019Article
- Characterization of Xenopus laevis guanine deaminase reveals new insights for its expression and function in the embryonic kidney.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Article
- Distinct Functions for Mammalian CLASP1 and -2 During Neurite and Axon Elongation.Frontiers in cellular neuroscience · 2019Article
- Human CLASP2 specifically regulates microtubule catastrophe and rescue.Molecular biology of the cell · 2018Article
- Motor axon navigation relies on Fidgetin-like 1-driven microtubule plus end dynamics.The Journal of cell biology · 2018Article
- The Role of the Microtubule Cytoskeleton in Neurodevelopmental Disorders.Frontiers in cellular neuroscience · 2018Review
- Article
- Neurite elongation is highly correlated with bulk forward translocation of microtubules.Scientific reports · 2017Article
- Xenopus laevis as a model system to study cytoskeletal dynamics during axon pathfinding.Genesis (New York, N.Y. : 2000) · 2017Review
- The Kinesin Adaptor Calsyntenin-1 Organizes Microtubule Polarity and Regulates Dynamics during Sensory Axon Arbor Development.Frontiers in cellular neuroscience · 2017Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Dynamic microtubules (MTs) are required for neuronal guidance, in which axons extend directionally toward their target tissues. We found that depletion of the MT-binding protein Xenopus cytoplasmic linker-associated protein 1 (XCLASP1) or treatment with the MT drug Taxol reduced axon outgrowth in spinal cord neurons. To quantify the dynamic distribution of MTs in axons, we developed an automated algorithm to detect and track MT plus ends that have been fluorescently labeled by end-binding protein 3 (EB3). XCLASP1 depletion reduced MT advance rates in neuronal growth cones, very much like treatment with Taxol, demonstrating a potential link between MT dynamics in the growth cone and axon extension. Automatic tracking of EB3 comets in different compartments revealed that MTs increasingly slowed as they passed from the axon shaft into the growth cone and filopodia. We used speckle microscopy to demonstrate that MTs experience retrograde flow at the leading edge. Microtubule advance in growth cone and filopodia was strongly reduced in XCLASP1-depleted axons as compared with control axons, but actin retrograde flow remained unchanged. Instead, we found that XCLASP1-depleted growth cones lacked lamellipodial actin organization characteristic of protrusion. Lamellipodial architecture depended on XCLASP1 and its capacity to associate with MTs, highlighting the importance of XCLASP1 in actin-microtubule interactions.
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