ArticleMolecular biology of the cell2013
Self-organization of stabilized microtubules by both spindle and midzone mechanisms in Xenopus egg cytosol.
Article in Molecular biology of the cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Force balance of opposing diffusive motors generates polarity-sorted microtubule patterns.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Extensile to contractile transition in active microtubule-actin composites generates layered asters with programmable lifetimes.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Active condensation of filaments under spatial confinement.Frontiers in physics · 2022Article
- The Generation of Dynein Networks by Multi-Layered Regulation and Their Implication in Cell Division.Frontiers in cell and developmental biology · 2020Review
- Proteomic Profiling of Microtubule Self-organization in M-phase.Molecular & cellular proteomics : MCP · 2018Article
- Emergent mechanics of actomyosin drive punctuated contractions and shape network morphology in the cell cortex.PLoS computational biology · 2018Article
- Prc1E and Kif4A control microtubule organization within and between largeMolecular biology of the cell · 2018Article
- Chromosomal passenger complex hydrodynamics suggests chaperoning of the inactive state by nucleoplasmin/nucleophosmin.Molecular biology of the cell · 2017Article
- Microtubule motors involved in nuclear movement during skeletal muscle differentiation.Molecular biology of the cell · 2017Article
- Spatial confinement of active microtubule networks induces large-scale rotational cytoplasmic flow.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Kif4 Is Essential for Mouse Oocyte Meiosis.PloS one · 2017Article
- Vertebrate Embryonic Cleavage Pattern Determination.Advances in experimental medicine and biology · 2017Review
- Active contraction of microtubule networks.eLife · 2015Article
- Spindle-to-cortex communication in cleaving, polyspermic Xenopus eggs.Molecular biology of the cell · 2015Article
- The microtubule cross-linker Feo controls the midzone stability, motor composition, and elongation of the anaphase B spindle in Drosophila embryos.Molecular biology of the cell · 2015Article
- Aurora A's Functions During Mitotic Exit: The Guess Who Game.Frontiers in oncology · 2015Review
- Spatial organization of cytokinesis signaling reconstituted in a cell-free system.Science (New York, N.Y.) · 2014Article
- Article
- Multiple mechanisms determine ER network morphology during the cell cycle in Xenopus egg extracts.The Journal of cell biology · 2013Article
- Active Microphase Separation in Mixtures of Microtubules and Tip-Accumulating Molecular Motors.Physical review. XArticle
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Previous study of self-organization of Taxol-stabilized microtubules into asters in Xenopus meiotic extracts revealed motor-dependent organizational mechanisms in the spindle. We revisit this approach using clarified cytosol with glycogen added back to supply energy and reducing equivalents. We added probes for NUMA and Aurora B to reveal microtubule polarity. Taxol and dimethyl sulfoxide promote rapid polymerization of microtubules that slowly self-organize into assemblies with a characteristic morphology consisting of paired lines or open circles of parallel bundles. Minus ends align in NUMA-containing foci on the outside, and plus ends in Aurora B-containing foci on the inside. Assemblies have a well-defined width that depends on initial assembly conditions, but microtubules within them have a broad length distribution. Electron microscopy shows that plus-end foci are coated with electron-dense material and resemble similar foci in monopolar midzones in cells. Functional tests show that two key spindle assembly factors, dynein and kinesin-5, act during assembly as they do in spindles, whereas two key midzone assembly factors, Aurora B and Kif4, act as they do in midzones. These data reveal the richness of self-organizing mechanisms that operate on microtubules after they polymerize in meiotic cytoplasm and provide a biochemically tractable system for investigating plus-end organization in midzones.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.