ArticleThe Journal of general virology2013
An essential RNA element resides in a central region of hepatitis E virus ORF2.
Article in The Journal of general virology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Article
- Plasmacytoid dendritic cell sensing of hepatitis E virus is shaped by both viral and host factors.Life science alliance · 2025Article
- Expanding the Hepatitis E Virus Toolbox: Selectable Replicons and Recombinant Reporter Genomes.Viruses · 2023Review
- Characteristics and Functions of HEV Proteins.Advances in experimental medicine and biology · 2023Article
- An RNA Interference/Adeno-Associated Virus Vector-Based Combinatorial Gene Therapy Approach Against Hepatitis E Virus.Hepatology communications · 2022Article
- Processing and Subcellular Localization of the Hepatitis E Virus Replicase: Identification of Candidate Viral Factories.Frontiers in microbiology · 2022Article
- In silico and in vitro screening of licensed antimalarial drugs for repurposing as inhibitors of hepatitis E virus.In silico pharmacology · 2021Article
- Review
- Proline-Rich Hypervariable Region of Hepatitis E Virus: Arranging the Disorder.Microorganisms · 2020Article
- Identification of functional cis-acting RNA elements in the hepatitis E virus genome required for viral replication.PLoS pathogens · 2020Article
- Article
- Stem Cell-Derived Culture Models of Hepatitis E Virus Infection.Cold Spring Harbor perspectives in medicine · 2019Review
- Roles of the genomic sequence surrounding the stem-loop structure in the junction region including the 3' terminus of open reading frame 1 in hepatitis E virus replication.Journal of medical virology · 2018Article
- Hepatitis E Virus Mutations: Functional and Clinical Relevance.EBioMedicine · 2016Review
- Hepatitis E virus and fulminant hepatitis--a virus or host-specific pathology?Liver international : official journal of the International Association for the Study of the Liver · 2015Article
- Therapeutic targets for the treatment of hepatitis E virus infection.Expert opinion on therapeutic targets · 2015Review
- Mapping overlapping functional elements embedded within the protein-coding regions of RNA viruses.Nucleic acids research · 2014Article
- Hepatitis E and lymphocytic leukemia in Man, Italy.Emerging infectious diseases · 2013Article
- Hepatitis E virus: foodborne, waterborne and zoonotic transmission.International journal of environmental research and public health · 2013Review
- Hepatitis e: molecular virology and pathogenesis.Journal of clinical and experimental hepatology · 2013Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Hepatitis E virus (genus Hepevirus, family Hepeviridae) is one of the most important causes of acute hepatitis in adults, particularly among pregnant women, throughout Asia and Africa where mortality rates can be 20-30 %. Hepatitis E virus has a single-stranded positive-sense RNA genome that contains three translated ORFs. The two 3' ORFs are translated from a subgenomic RNA. Functional RNA elements have been identified in and adjacent to the genomic 5' and 3' UTRs and in and around the intergenic region. Here we describe an additional RNA element that is located in a central region of ORF2. The RNA element is predicted to fold into two highly conserved stem-loop structures, ISL1 and ISL2. Mutations that disrupt the predicted structures, without altering the encoded amino acid sequence, result in a drastic reduction in capsid protein synthesis. This indicates that the RNA element plays an important role in one of the early steps of virus replication. The structures were further investigated using a replicon that expresses Gaussia luciferase in place of the capsid protein. Single mutations in ISL2 severely reduced luciferase expression, but a pair of compensatory mutations that were predicted to restore the ISL2 structure, restored luciferase expression to near-WT levels, thus lending experimental support to the predicted structure. Nonetheless the precise role of the ISL1+ISL2 element remains unknown.
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