ArticleJournal of the American Chemical Society2013
Colocalization of the ganglioside G(M1) and cholesterol detected by secondary ion mass spectrometry.
Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 73 citations in OpenAlex.
- ToF-SIMS Imaging for the Analysis of Cholesterol Formation at Macrophage Membrane.Metabolites · 2025Article
- Biomembrane structure at the molecular level and its application in precision medicine.Biophysics reviews · 2025Review
- Secondary Ion Mass Spectrometry of Single Giant Unilamellar Vesicles Reveals Compositional Variability.Journal of the American Chemical Society · 2023Article
- Bacterial toxins and heart function: heat-labileBiophysical reviews · 2023Article
- Imaging and Manipulation of Plasma Membrane Fatty Acid Clusters Using TOF-SIMS Combined Optogenetics.Cells · 2022Article
- Recombination betweenAnalytical chemistry · 2022Article
- Review
- A Trifunctional Linker for Palmitoylation and Peptide and Protein Localization in Biological Membranes.Chembiochem : a European journal of chemical biology · 2020Article
- DNA nanotweezers for stabilizing and dynamically lighting up a lipid raft on living cell membranes and the activation of T cells.Chemical science · 2020Article
- Membrane Dynamics in Health and Disease: Impact on Cellular Signalling.The Journal of membrane biology · 2019Review
- Imaging with mass spectrometry, the next frontier in sphingolipid research? A discussion on where we stand and the possibilities ahead.Chemistry and physics of lipids · 2019Review
- Cholesterol-pyrene as a probe for cholesterol distribution on ordered and disordered membranes: Determination of spectral wavelengths.PloS one · 2018Article
- Emphatic visualization of sphingomyelin-rich domains by inter-lipid FRET imaging using fluorescent sphingomyelins.Scientific reports · 2017Article
- Diffusion of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles measured by STED-FCS.Molecular biology of the cell · 2017Article
- The mystery of membrane organization: composition, regulation and roles of lipid rafts.Nature reviews. Molecular cell biology · 2017Review
- Atomic Recombination in Dynamic Secondary Ion Mass Spectrometry Probes Distance in Lipid Assemblies: A Nanometer Chemical Ruler.Journal of the American Chemical Society · 2016Article
- Dynamic Reorganization and Correlation among Lipid Raft Components.Journal of the American Chemical Society · 2016Article
- Nonenzymatic biomimetic remodeling of phospholipids in synthetic liposomes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- A comparative study on fluorescent cholesterol analogs as versatile cellular reporters.Journal of lipid research · 2016Article
- Sphingolipid Organization in the Plasma Membrane and the Mechanisms That Influence It.Frontiers in cell and developmental biology · 2016Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The characterization of the lateral organization of components in biological membranes and the evolution of this arrangement in response to external triggers remain a major challenge. The concept of lipid rafts is widely invoked; however, direct evidence of the existence of these ephemeral entities remains elusive. We report here the use of secondary ion mass spectrometry (SIMS) to image the cholesterol-dependent cohesive phase separation of the ganglioside GM1 into nano- and microscale assemblies in a canonical lipid raft composition of lipids. This assembly of domains was interrogated in a model membrane system composed of palmitoyl sphingomyelin (PSM), cholesterol, and an unsaturated lipid (dioleoylphosphatidylcholine, DOPC). Orthogonal isotopic labeling of every lipid bilayer component and monofluorination of GM1 allowed generation of molecule specific images using a NanoSIMS. Simultaneous detection of six different ion species in SIMS, including secondary electrons, was used to generate ion ratio images whose signal intensity values could be correlated to composition through the use of calibration curves from standard samples. Images of this system provide the first direct, molecule specific, visual evidence for the colocalization of cholesterol and GM1 in supported lipid bilayers and further indicate the presence of three compositionally distinct phases: (1) the interdomain region; (2) micrometer-scale domains (d > 3 μm); (3) nanometer-scale domains (d = 100 nm to 1 μm) localized within the micrometer-scale domains and the interdomain region. PSM-rich, nanometer-scale domains prefer to partition within the more ordered, cholesterol-rich/DOPC-poor/GM1-rich micrometer-scale phase, while GM1-rich, nanometer-scale domains prefer to partition within the surrounding, disordered, cholesterol-poor/PSM-rich/DOPC-rich interdomain phase.
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