ArticleBMC veterinary research2013
Species-specific multiplex PCR for the diagnosis of Brucella ovis, Actinobacillus seminis, and Histophilus somni infection in rams.
Article in BMC veterinary research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Addition of lactic acid bacteria to diluted ram semen as vehicle for vaginal inoculation: interaction with seminal microbiota, sperm quality and antibacterial in vitro effect against Mycoplasma agalactiae.BMC veterinary research · 2026Article
- Metabarcoding analysis of the microbiota in flocks naturally infected byOne health (Amsterdam, Netherlands) · 2025Article
- Identification ofItalian journal of food safety · 2025Article
- Simultaneous and rapid detection of avian respiratory diseases of small poultry using multiplex reverse transcription-Polymerase Chain Reaction assay.Poultry science · 2023Article
- Establishment and Application of a Multiplex PCR Assay for Detection of Sclerotium rolfsii, Lasiodiplodia theobromae, and Fusarium oxysporum in Peanut.Molecular biotechnology · 2023Article
- Comparative Review of Brucellosis in Small Domestic Ruminants.Frontiers in veterinary science · 2022Review
- Influence of the Ovine Genital Tract Microbiota on the Species Artificial Insemination Outcome. A Pilot Study in Commercial Sheep Farms.High-throughput · 2020Article
- Development and application of multiplex PCR method for simultaneous detection of seven viruses in ducks.BMC veterinary research · 2019Article
- Evaluation of serologic testing of rams in the management of Brucella ovis in a domestic sheep flock.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019Article
- Histophilosis as a Natural Disease.Current topics in microbiology and immunology · 2016Review
- Detection of antibodies to the biofilm exopolysaccharide of Histophilus somni following infection in cattle by enzyme-linked immunosorbent assay.Clinical and vaccine immunology : CVI · 2014Article
- Clinical and pathological changes in rams experimentally infected with Actinobacillus seminis and Histophilus somni.TheScientificWorldJournal · 2014Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInfectious ovine epididymitis results in substantial economic losses worldwide due to reproductive failure and culling of breeders. The most common causative agents of these infections are Brucella ovis, Actinobacillus seminis, and Histophilus somni. The aim of this study was to develop a multiplex PCR assay for simultaneous detection of Brucella ovis, Actinobacillus seminis, and Histophilus somni with species-specific primers applied to biological samples for molecular diagnosis of these infections.
resultsThe multiplex assay was capable of detecting B. ovis, A. seminis, and H. somni DNA simultaneously from genomic bacterial DNA samples and pool of semen samples from experimentally infected rams. The method was highly specific since it did not amplify DNA from other bacterial species that can potentially cause epididymitis in rams as well as species phylogenetically related to B. ovis. All negative control samples were negative in PCR multiplex assay. Urine can be used as an alternative to semen samples.
conclusionsThe species-specific multiplex PCR assay developed in this study can be successfully used for the detection of three of the most common bacterial causes of ovine epididymitis.
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