ArticleProceedings of the National Academy of Sciences of the United States of America2013
Single-cell imaging of HIV-1 provirus (SCIP).
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- ERVK activity in CD8Journal of neuroinflammation · 2026Article
- Clicking viruses-with chemistry toward mechanisms in infection.Journal of virology · 2025Review
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- Factors that mold the nuclear landscape of HIV-1 integration.Nucleic acids research · 2021Review
- Measuring the subcellular compartmentalization of viral infections by protein complementation assay.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Towards a Functional Cure of HIV-1: Insight Into the Chromatin Landscape of the Provirus.Frontiers in microbiology · 2021Review
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- A small targeting domain in Ty1 integrase is sufficient to direct retrotransposon integration upstream of tRNA genes.The EMBO journal · 2020Article
- The chromatin landscape at the HIV-1 provirus integration site determines viral expression.Nucleic acids research · 2020Article
- HIV-1 uncoats in the nucleus near sites of integration.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Single-Cell Technologies Applied to HIV-1 Research: Reaching Maturity.Frontiers in microbiology · 2020Review
- Spatially clustered loci with multiple enhancers are frequent targets of HIV-1 integration.Nature communications · 2019Article
- Design of reverse transcriptase-specific nucleosides to visualize early steps of HIV-1 replication by click labeling.The Journal of biological chemistry · 2019Article
- Capsid-CPSF6 interaction: Master regulator of nuclear HIV-1 positioning and integration.Journal of life sciences (Westlake Village, Calif.) · 2019Article
- Impact of LEDGIN treatment during virus production on residual HIV-1 transcription.Retrovirology · 2019Article
- The role of integration and clonal expansion in HIV infection: live long and prosper.Retrovirology · 2018Review
- Capsid-CPSF6 Interaction Licenses Nuclear HIV-1 Trafficking to Sites of Viral DNA Integration.Cell host & microbe · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent advances in fluorescence microscopy provided tools for the investigation and the analysis of the viral replication steps in the cellular context. In the HIV field, the current visualization systems successfully achieve the fluorescent labeling of the viral envelope and proteins, but not the genome. Here, we developed a system able to visualize the proviral DNA of HIV-1 through immunofluorescence detection of repair foci for DNA double-strand breaks specifically induced in the viral genome by the heterologous expression of the I-SceI endonuclease. The system for Single-Cell Imaging of HIV-1 Provirus, named SCIP, provides the possibility to individually track integrated-viral DNA within the nuclei of infected cells. In particular, SCIP allowed us to perform a topological analysis of integrated viral DNA revealing that HIV-1 preferentially integrates in the chromatin localized at the periphery of the nuclei.
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