Evidence map›Paper›PMID 23512538›Full record

ArticleThe Journal of pharmacology and experimental therapeutics2013

The tumor suppressor Kruppel-like factor 6 is a novel aryl hydrocarbon receptor DNA binding partner.

Shelly R Wilson, Aditya D Joshi, Cornelis J Elferink

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of pharmacology and experimental therapeutics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers.

0numbers the graph read from it
0cells of the map it votes in
92citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

92 citing papers in PubMed, 129 citations in OpenAlex.

  1. Review
  2. Review
  3. Functional genomic analysis of non-canonical DNA regulatory elements of the aryl hydrocarbon receptor.Toxicological sciences : an official journal of the Society of Toxicology · 2026
    Article
  4. Review
  5. Review
  6. Review
  7. Article
  8. Article
  9. Review
  10. Review
  11. The aryl hydrocarbon receptor: a new frontier in male reproductive system.Reproductive biology and endocrinology : RB&E · 2025
    Review
  12. Article
  13. AhR and STAT3: A Dangerous Duo in Chemical Carcinogenesis.International journal of molecular sciences · 2025
    Review
  14. Article
  15. Article
  16. Review
  17. Article
  18. Review
  19. Review
  20. Article

32 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Shelly R WilsonDepartment of Pharmacology and Toxicology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX, USA.
Aditya D Joshi
Cornelis J Elferink
The University of Texas Medical Branch at Galveston · US

Funding

Synthetic Organic Chemistry CoreP30ES006676 · NIEHS · UNIVERSITY OF TEXAS MEDICAL BR GALVESTON · PI ELFERINK, CORNELIS JOHAN · 1995 to 2017
$21.7M
Ah Receptor Anatomy: Implications for Dioxin ToxicityR01ES007800 · NIEHS · UNIVERSITY OF TEXAS MEDICAL BR GALVESTON · PI ELFERINK, CORNELIS JOHAN · 2001 to 2011
$3.5M
Ah receptor transcriptional regulation through a novel DNA binding siteF30ES016490 · NIEHS · UNIVERSITY OF TEXAS MED BR GALVESTON · PI WILSON, SHELLY RENEE · 2008 to 2012
$142k
NIEHS NIH HHS F30 ES016490NIEHS NIH HHS F30ES016490NIEHS NIH HHS P30 ES006676NIEHS NIH HHS P30ES006676NIEHS NIH HHS R01 ES007800NIEHS NIH HHS R01ES007800
6 · The paper itself

Abstract

The aryl hydrocarbon receptor (AhR) is a ligand-mediated basic helix-loop-helix transcription factor of the Per/Arnt/Sim family that regulates adaptive and toxic responses to a variety of chemical pollutants, including polycyclic aromatic hydrocarbons and halogenated aromatic hydrocarbons, most notably 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Ligand activation leads to AhR nuclear translocation and binding to a xenobiotic response element (XRE) in association with the Arnt to regulate gene expression. Several recent genome-wide transcriptional studies identified numerous AhR target genes that lack the canonical XRE recognition site in the promoter regions. Characterization of one such target gene, the plasminogen activator inhibitor 1, identified a novel nonconsensus XRE (NC-XRE) that confers TCDD responsiveness independently of the Arnt protein. Studies reported here show that the NC-XRE is a recognition site for the AhR and a new binding partner, the Kruppel-like factor (KLF) family member KLF6. In vivo chromatin immunoprecipitations and in vitro DNA binding studies demonstrate that the AhR and KLF6 proteins form an obligatory heterodimer necessary for NC-XRE binding. Mutational analyses show that the protein-protein interactions involve the AhR C terminus and KLF6 N terminus, respectively. Moreover, NC-XRE binding depends on the 5' basic region in KLF6 rather than the previously characterized zinc finger DNA binding domain. Collectively, the results unmask a novel AhR signaling mechanism distinct from the canonical XRE-driven process that will enrich our future understanding of AhR biology.

Indexed as

AnimalsBlotting, WesternCell NucleusChromatin ImmunoprecipitationDNAElectrophoretic Mobility Shift AssayEnvironmental PollutantsFemaleHumansKruppel-Like Factor 6Kruppel-Like Transcription FactorsMiceMice, Inbred C57BLMice, TransgenicPolychlorinated DibenzodioxinsPromoter Regions, GeneticDNAEnvironmental PollutantsKLF6 protein, humanKruppel-Like Factor 6Kruppel-Like Transcription FactorsPolychlorinated DibenzodioxinsProto-Oncogene ProteinsReceptors, Aryl HydrocarbonRNA-Binding ProteinsSERBP1 protein, humanXenobiotics

Identifiers

PMID23512538
PMCPMC3657114
OpenAlexW1964552446

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.