ArticleNature communications2013
Impact of macromolecular crowding on DNA replication.
Article in Nature communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
74 citing papers in PubMed, 175 citations in OpenAlex.
- High-throughput identification of endogenous biomolecular condensates and phase-separating proteins.Nature protocols · 2026Review
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- Scaling laws in confined media applied for biomarker detection.Nature communications · 2026Article
- How does cytoplasmic crowding affect reaction rates?Molecular cell · 2026Review
- Maintenance of cytoplasmic and membrane densities shapes cellular geometry in Escherichia coli.Nature communications · 2025Article
- Osmotic pressure regulates DNA labelling and transcription with dCas9-SunTag system in live cells.Nature communications · 2025Article
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- Characterization of DnaB-DnaG Interaction in M. tuberculosis Using Small-Angle X-ray Scattering-Based Dissociation Assay.Chembiochem : a European journal of chemical biology · 2025Article
- Underestimated role of macromolecular crowding in bioengineeredMaterials today. Bio · 2025Article
- Melting Profile of DNA in Crowded Solution: Model-Based Study.International journal of molecular sciences · 2025Article
- Ultrasensitive CRISPR/Cas12a-Based System for Detection ofCurrent issues in molecular biology · 2025Article
- Elucidating the Role of Groove Hydration on Stability and Functions of Biased DNA Duplexes in Cell-Like Chemical Environments.Journal of the American Chemical Society · 2024Article
- Hypothesis: bacteria live on the edge of phase transitions with a cell cycle regulated by a water-clock.Theory in biosciences = Theorie in den Biowissenschaften · 2024Article
- Similarity Metrics for Subcellular Analysis of FRET Microscopy Videos.The journal of physical chemistry. B · 2024Article
- Cell size regulates human endoderm specification through actomyosin-dependent AMOT-YAP signaling.Stem cell reports · 2024Article
- High-throughput and proteome-wide discovery of endogenous biomolecular condensates.Nature chemistry · 2024Article
- Crowder titrations enable the quantification of driving forces for macromolecular phase separation.Biophysical journal · 2024Article
- Macromolecular Crowding, Phase Separation, and Homeostasis in the Orchestration of Bacterial Cellular Functions.Chemical reviews · 2024Review
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Enzymatic activities in vivo occur in a crowded environment composed of many macromolecules. This environment influences DNA replication by increasing the concentration of the constituents, desolvation, decreasing the degrees of freedom for diffusion and hopping of proteins onto DNA, and enhancing binding equilibria and catalysis. However, the effect of macromolecular crowding on protein structure is poorly understood. Here we examine macromolecular crowding using the replication system of bacteriophage T7 and we show that it affects several aspects of DNA replication; the activity of DNA helicase increases and the sensitivity of DNA polymerase to salt is reduced. We also demonstrate, using small-angle X-ray scattering analysis, that the complex between DNA helicase and DNA polymerase/trx is far more compact in a crowded environment. The highest enzymatic activity corresponds to the most compact structure. Better knowledge of the effect of crowding on structure and activity will enhance mechanistic insight beyond information obtained from NMR and X-ray structures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.