ArticleBMC plant biology2013
Overexpression of the transcription factor RAP2.6 leads to enhanced callose deposition in syncytia and enhanced resistance against the beet cyst nematode Heterodera schachtii in Arabidopsis roots.
Article in BMC plant biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 89 citations in OpenAlex.
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- Biochemical Defence of Plants against Parasitic Nematodes.Plants (Basel, Switzerland) · 2024Review
- Impact of Rhg1 copy number variation on a soybean cyst nematode resistance transcriptional network.G3 (Bethesda, Md.) · 2024Article
- Comparative transcriptional analysis of Persea americana MYB, WRKY and AP2/ERF transcription factors following Phytophthora cinnamomi infection.Molecular plant pathology · 2024Article
- Identification of resistant sources fromFrontiers in plant science · 2023Article
- Comparative Seeds Storage Transcriptome Analysis ofInternational journal of molecular sciences · 2022Article
- Integrated Omic Approaches Reveal Molecular Mechanisms of Tolerance during Soybean andPlants (Basel, Switzerland) · 2022Article
- The AP2/ERF GmERF113 Positively Regulates the Drought Response by ActivatingInternational journal of molecular sciences · 2022Article
- Sandwich Enzyme-Linked Immunosorbent Assay for Quantification of Callose.Methods and protocols · 2022Article
- Quantitative detection of induced systemic resistance genes of potato roots upon ethylene treatment and cyst nematode,Saudi journal of biological sciences · 2022Article
- Full-Length Transcriptional Analysis of the Same Soybean Genotype With Compatible and Incompatible Reactions toFrontiers in plant science · 2022Article
- WIND transcription factors orchestrate wound-induced callus formation, vascular reconnection and defense response in Arabidopsis.The New phytologist · 2021Article
- EgJUB1 and EgERF113 transcription factors as potential master regulators of defense response in Elaeis guineensis against the hemibiotrophic Ganoderma boninense.BMC plant biology · 2021Article
- Transcriptome Analysis Reveals the Important Role ofFrontiers in plant science · 2021Article
- Molecular insights into the compatible and incompatible interactions between sugar beet and the beet cyst nematode.BMC plant biology · 2020Article
- The Arabidopsis RboHB Encoded byInternational journal of molecular sciences · 2020Article
- The Arabidopsis GPI-Anchored LTPg5 Encoded byInternational journal of molecular sciences · 2020Article
- Early transcriptional responses to soybean cyst nematode HG Type 0 show genetic differences among resistant and susceptible soybeans.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundCyst nematodes invade the roots of their host plants as second stage juveniles and induce a syncytium which is their source of nutrients throughout their life. A transcriptome analysis of syncytia induced by the beet cyst nematode Heterodera schachtii in Arabidopsis roots has shown that gene expression in the syncytium is different from that of the root with thousands of genes upregulated or downregulated. Among the downregulated genes are many which code for defense-related proteins. One gene which is strongly downregulated codes for the ethylene response transcription factor RAP2.6. The genome of Arabidopsis contains 122 ERF transcription factor genes which are involved in a variety of developmental and stress responses.
resultsExpression of RAP2.6 was studied with RT-PCR and a promoter::GUS line. During normal growth conditions the gene was expressed especially in roots and stems. It was inducible by Pseudomonas syringae but downregulated in syncytia from a very early time point on. Overexpression of the gene enhanced the resistance against H. schachtii which was seen by a lower number of nematodes developing on these plants as well as smaller syncytia and smaller female nematodes. A T-DNA mutant had a reduced RAP2.6 transcript level but this did not further increase the susceptibility against H. schachtii. Neither overexpression lines nor mutants had an effect on P. syringae. Overexpression of RAP2.6 led to an elevated expression of JA-responsive genes during early time points after infection by H. schachtii. Syncytia developing on overexpression lines showed enhanced deposition of callose.
conclusionsOur results showed that H. schachtii infection is accompanied by a downregulation of RAP2.6. It seems likely that the nematodes use effectors to actively downregulate the expression of this and other defense-related genes to avoid resistance responses of the host plant. Enhanced resistance of RAP2.6 overexpression lines seemed to be due to enhanced callose deposition at syncytia which might interfere with nutrient import into syncytia.
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