ArticleBMC genomics2013
Functional effect of mir-27b on myostatin expression: a relationship in Piedmontese cattle with double-muscled phenotype.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 54 citations in OpenAlex.
- Welfare of beef cattle.EFSA journal. European Food Safety Authority · 2025Article
- Bio-loggers and miRNAs are innovative tools for measuring physiological changes in lambs during transport.Journal of animal science · 2025Article
- MicroRNAs and their Modulatory Effect on the Hallmarks of Osteosarcopenia.Current osteoporosis reports · 2024Review
- Circulating skeletal muscle related microRNAs profile in Piedmontese cattle during different age.Scientific reports · 2021Article
- Comparative Genome and Transcriptome Integration Studies Reveal the Mechanism of Pectoral Muscle Development and Function in Pigeons.Frontiers in genetics · 2021Article
- Article
- MicroRNA-33a negatively regulates myoblast proliferation by targeting IGF1, follistatin and cyclin D1.Bioscience reports · 2020Article
- miR-181b-5p May Regulate Muscle Growth in Tilapia by Targeting Myostatin b.Frontiers in endocrinology · 2019Article
- Dyslipidemia Is a Major Factor in Stem Cell Damage Induced by Uncontrolled Long-Term Type 2 Diabetes and Obesity in the Rat, as Suggested by the Effects on Stem Cell Culture.The journal of sexual medicine · 2018Article
- MiR-27b promotes sheep skeletal muscle satellite cell proliferation by targetingJournal of genetics · 2018Article
- miR-27b regulates myogenic proliferation and differentiation by targeting Pax3 in goat.Scientific reports · 2018Article
- Article
- Micromanagement of Immune System: Role of miRNAs in Helminthic Infections.Frontiers in microbiology · 2017Review
- miR-378a-3p promotes differentiation and inhibits proliferation of myoblasts by targeting HDAC4 in skeletal muscle development.RNA biology · 2016Article
- Effect of differentiation on microRNA expression in bovine skeletal muscle satellite cells by deep sequencing.Cellular & molecular biology letters · 2016Article
- Article
- The miRNA Transcriptome Directly Reflects the Physiological and Biochemical Differences between Red, White, and Intermediate Muscle Fiber Types.International journal of molecular sciences · 2015Article
- RNA sequencing identifies upregulated kyphoscoliosis peptidase and phosphatidic acid signaling pathways in muscle hypertrophy generated by transgenic expression of myostatin propeptide.International journal of molecular sciences · 2015Article
- Identification and analysis of genetic variations in pri-miRNAs expressed specifically or at a high level in sheep skeletal muscle.PloS one · 2015Article
- Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn Piedmontese cattle the double-muscled phenotype is an inherited condition associated to a point mutation in the myostatin (MSTN) gene. The Piedmontese MSTN missense mutation G938A is translated to C313Y myostatin protein. This mutation alters MSTN function as a negative regulator of muscle growth, thereby inducing muscle hypertrophy. MiRNAs could play a role in skeletal muscle hypertrophy modulation by down-regulating gene expression.
resultsAfter identifying a 3'-UTR consensus sequence of several negative and positive modulator genes involved in the skeletal muscle hypertrophy pathway, such as IGF1, IGF1R, PPP3CA, NFATc1, MEF2C, GSK3b, TEAD1 and MSTN, we screened miRNAs matching to it. This analysis led to the identification of miR-27b, miR-132, miR-186 and miR-199b-5p as possible candidates. We collected samples of longissimus thoracis from twenty Piedmontese and twenty Friesian male bovines. In Piedmontese group miR-27b was up-regulated 7.4-fold (p < 0.05). Further, we report that the level of MSTN mRNA was about 5-fold lower in Piedmontese cattle vs Friesian cattle (p < 0.0001) and that less mature MSTN protein was detected in the Piedmontese one (p < 0.0001). Cotransfection of miR-27b and psi-check2 vector with the luciferase reporter gene linked to the bovine wild-type 3'-UTR of MSTN strongly inhibited the luciferase activity (79%, p < 0.0001).
conclusionsThese data demonstrate that bovine MSTN is a specific target of miR-27b and that miRNAs contribute to explain additive phenotypic hypertrophy in Piedmontese cattle selected for the MSTN gene mutation, possibly outlining a more precise genetic signature able to elucidate differences in muscle conformation.
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