ArticleBioMed research international2013
Loop-mediated isothermal amplification assay for the rapid detection of Staphylococcus aureus.
Article in BioMed research international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Evaluation of the loop-mediated isothermal amplification assay for Staphylococcus aureus detection: a systematic review and meta-analysis.Annals of clinical microbiology and antimicrobials · 2022Pooled it
- Basal foraminifer endures anoxia via aerotolerant anaerobic mitochondria and unconventional energy metabolism.The ISME journal · 2026Article
- Development of Duplex Loop-Mediated Isothermal Amplification with Hydroxynaphthol Blue for Detection of Infectious Spleen and Kidney Necrosis Virus andMicroorganisms · 2025Article
- A novel extraction-free dual HiFi-LAMP assay for detection of methicillin-sensitive and methicillin-resistantMicrobiology spectrum · 2024Article
- Rapid Visual Detection of Methicillin-ResistantMicroorganisms · 2024Article
- Loop-Mediated Isothermal Amplification of DNA (LAMP) as an Alternative Method for Determining Bacteria in Wound Infections.International journal of molecular sciences · 2023Review
- Novel helix loop-mediated isothermal amplification (HAMP) assay for colorimetric detection of Staphylococcus aureus in milk.World journal of microbiology & biotechnology · 2023Article
- The application of the loop-mediated isothermal amplification method for rapid detection of methicillin-resistantNew microbes and new infections · 2022Article
- Article
- Evaluation of bronchoalveolar lavage fluid combined with the loop-mediated isothermal amplification assay in lower respiratory tract infections.American journal of translational research · 2020Article
- Detection of Potato ring rot Pathogen Clavibacter michiganensis subsp. sepedonicus by Loop-mediated isothermal amplification (LAMP) assay.Scientific reports · 2019Article
- Loop-mediated isothermal amplification assays for Enterococcussp., Escherichiacoli and Staphylococcusaureus in chicken.FEMS microbiology letters · 2019Article
- Simple Visualized Detection Method of Virulence-Associated Genes ofFrontiers in microbiology · 2019Article
- Development and Evaluation of Simple Dot-Blot Assays for Rapid Detection of Staphylococcal Enterotoxin-A in Food.Indian journal of microbiology · 2017Article
- Article
- The use of loop-mediated isothermal DNA amplification for the detection and identification of the anthrax pathogen.Molecular genetics, microbiology and virology : Molekulyarnaya genetika, mikrobiologiya i virusologiya · 2017Article
- Article
- Development and Diagnostic Evaluation of Loop-Mediated Isothermal Amplification Using a New Gene Target for Rapid Detection of Helicobacter pylori.Jundishapur journal of microbiology · 2016Article
- PCR-based Approaches for the Detection of Clinical Methicillin-resistant Staphylococcus aureus.The open microbiology journal · 2016Article
- Loop-Mediated Isothermal Amplification for Laboratory Confirmation of Buruli Ulcer Disease-Towards a Point-of-Care Test.PLoS neglected tropical diseases · 2015Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA), is an important human pathogen that produces a variety of toxins and causes a wide range of infections, including soft-tissue infections, bacteremia, and staphylococcal food poisoning. A loop-mediated isothermal amplification (LAMP) assay targeting the arcC gene of S. aureus was developed and evaluated with 119 S. aureus and 25 non-S. aureus strains. The usefulness of the assay was compared with the PCR method that targets spa and arcC genes. The optimal temperature for the LAMP assay was 58.5°C with a detection limit of 2.5 ng/μL and 10(2) CFU/mL when compared to 12.5 ng/μL and 10(3) CFU/mL for PCR (spa and arcC). Both LAMP and PCR assays were 100% specific, 100% sensitive, 100% positive predictive value (PPV), and 100% negative predictive value (NPV). When tested on 30 spiked blood specimens (21 MRSA, eight non-S. aureus and one negative control), the performance of LAMP and PCR was comparable: 100% specific, 100% sensitive, 100% PPV, and 100% NPV. In conclusion, the LAMP assay was equally specific with a shorter detection time when compared to PCR in the identification of S. aureus. The LAMP assay is a promising alternative method for the rapid identification of S. aureus and could be used in resource-limited laboratories and fields.
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