ArticleBioMed research international2013
Cytotoxic effect of ethanol extract of microalga, Chaetoceros calcitrans, and its mechanisms in inducing apoptosis in human breast cancer cell line.
Article in BioMed research international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
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24 citing papers in PubMed, 59 citations in OpenAlex.
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- Microalgae and Human Disorders: Mechanistic Insights and Therapeutic Potential.Current topics in medicinal chemistry · 2025Review
- Screening of Microalgae for Bioactivity with Antiviral, Antibacterial, Anti-Inflammatory and Anti-Cancer Assays.Biology · 2024Article
- Natural Gallic Acid and Methyl Gallate Induces Apoptosis in Hela Cells through Regulation of Intrinsic and Extrinsic Protein Expression.International journal of molecular sciences · 2023Article
- Cytotoxicity andHeliyon · 2022Article
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- Anticancer Compounds Derived from Marine Diatoms.Marine drugs · 2020Review
- Meeting Sustainable Development Goals: Alternative Extraction Processes for Fucoxanthin in Algae.Frontiers in bioengineering and biotechnology · 2020Review
- Monoacylglycerides from the DiatomMarine drugs · 2019Article
- Increased fucoxanthin inBiotechnology reports (Amsterdam, Netherlands) · 2019Article
- Marine Microalgae with Anti-Cancer Properties.Marine drugs · 2018Review
- Metabolite Profiling of the Microalgal DiatomMarine drugs · 2018Article
- First identification of marine diatoms with anti-tuberculosis activity.Scientific reports · 2018Article
- Effect of Enzymatic pre-treatment of microalgae extracts on their anti-tumor activity.Biomedical journal · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Marine microalgae have been prominently featured in cancer research. Here, we examined cytotoxic effect and apoptosis mechanism of crude ethanol extracts of an indigenous microalga, Chaetoceros calcitrans (UPMAAHU10) on human breast cell lines. MCF-7 was more sensitive than MCF-10A with IC50 value of 3.00 ± 0.65, whilst the IC50 value of Tamoxifen against MCF-7 was 12.00 ± 0.52 μg/mL after 24 hour incubation. Based on Annexin V/Propidium iodide and cell cycle flow cytometry analysis, it was found that inhibition of cell growth by EEC on MCF-7 cells was through the induction of apoptosis without cell cycle arrest. The apoptotic cells at subG0/G1 phase in treated MCF-7 cells at 48 and 72 hours showed 34 and 16 folds increased compared to extract treated MCF-10A cells which showed only 6 and 7 folds increased at the same time points, respectively. Based on GeXP study, EEC induced apoptosis on MCF-7 cells via modulation of CDK2, MDM2, p21Cip1, Cyclin A2, Bax and Bcl-2. The EEC treated MCF-7 cells also showed an increase in Bax/Bcl-2 ratio that in turn activated the caspase-dependent pathways by activating caspase 7. Thus, marine microalga, Chaetoceros calcitrans may be considered a good candidate to be developed as a new anti-breast cancer drug.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.