ArticleBioMed research international2013
Differentiation of induced pluripotent stem cells into male germ cells in vitro through embryoid body formation and retinoic acid or testosterone induction.
Article in BioMed research international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Promoting mBMMSC differentiation into late-stage germ-like cells through retinoic acid, fibrin-coated titanium nanotubes, and ultraviolet radiation.Histochemistry and cell biology · 2025Article
- The impact of induced pluripotent stem cells in animal conservation.Veterinary research communications · 2024Review
- Investigation of Signals and Transcription Factors for The Generation of Female Germ-Like Cells.Cell journal · 2022Article
- Resurrecting biodiversity: advanced assisted reproductive technologies and biobanking.Reproduction & fertility · 2022Review
- Primordial germ cells can be differentiated by retinoic acid and progesterone induction from embryonic stem cells.Journal of biosciences · 2021Article
- Induced pluripotent stem cell: A headway in reprogramming with promising approach in regenerative biology.Veterinary world · 2017Review
- In vitro generation of Sertoli-like and haploid spermatid-like cells from human umbilical cord perivascular cells.Stem cell research & therapy · 2017Article
- CHIR99021 combined with retinoic acid promotes the differentiation of primordial germ cells from human embryonic stem cells.Oncotarget · 2017Article
- Reversine Increases the Plasticity of Long-Term Cryopreserved Fibroblasts to Multipotent Progenitor Cells through Activation of Oct4.International journal of biological sciences · 2016Article
- Human iPS Cell-Derived Germ Cells: Current Status and Clinical Potential.Journal of clinical medicine · 2014Review
- Potential spermatogenesis recovery with bone marrow mesenchymal stem cells in an azoospermic rat model.International journal of molecular sciences · 2014Article
- Stem cells as new agents for the treatment of infertility: current and future perspectives and challenges.BioMed research international · 2014Review
- Generation of male germ cellsAsian journal of andrologyReview
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Generation of germ cells from pluripotent stem cells in vitro could have great application for treating infertility and provides an excellent model for uncovering molecular mechanisms controlling gametogenesis. In this study, we explored the differentiation potential of mouse induced pluripotent stem (iPS) cells towards male germ cells. Embryoid body formation and retinoic acid/testosterone induction were applied to promote differentiation of mouse iPS cells into male germ cells in vitro. Quantitative RT-PCR and immunoflourescence were performed to characterize the iPS cell differentiation process, and notably there were different temporal expression profiles of male germ cell-associated genes. The expression of proteins, including MVH, CDH1, and SCP3, was remarkably increased. mRNA expression of Stra8, Odf2, Act, and Prm1 was upregulated in iPS cells by retinoic acid or testosterone induction, whereas Oct-4 transcription was reduced in these cells compared to the controls. Hormones were also measured in the EB medium. DNA content analysis by flow cytometry revealed that iPS cells could differentiate into haploid cells through retinoic acid or testosterone treatment. Collectively, our results suggest that mouse iPS cells possess the potency to differentiate into male germ cells in vitro through embryoid body formation and retinoic acid or testosterone induction.
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