ArticleBioMed research international2013
A pentaplex PCR assay for the detection and differentiation of Shigella species.
Article in BioMed research international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Comparative Evaluation of Rapid Nucleic Acids Extraction Methods for Biosensor-Based Point-of-Care Solutions.Biosensors · 2026Article
- "Molecular Characterization of Extended Spectrum Beta-Lactamase Resistance in Pediatric Shigella Isolates in Egypt".Biochemical genetics · 2025Article
- A Perspective on Wastewater and Environmental Surveillance as a Public Health Tool for Low- and Middle-Income Countries.Microorganisms · 2025Article
- Investigation of the vaccine potential of anVaccine: X · 2024Article
- Characterization of genotypes and antimicrobial resistance profiles of clinical isolates of Shigella from patients in the southern region of Iran.European journal of medical research · 2023Article
- A novel approach for simultaneous detection of the most common food-borne pathogens by multiplex qPCR.Biomolecules & biomedicine · 2023Article
- Isolation and Molecular Identification ofArchives of Razi Institute · 2023Article
- Occurrence of shigellosis in pediatric diarrheal patients in Chattogram, Bangladesh: A molecular based approach.PloS one · 2023Article
- Molecular detection and characterization of Shigella spp. harboring extended-spectrum β-lactamase genes in children with diarrhea in northwest Iran.Molecular and cellular pediatrics · 2022Article
- The Detection of Foodborne Pathogenic Bacteria in Seafood Using a Multiplex Polymerase Chain Reaction System.Foods (Basel, Switzerland) · 2022Article
- Development and Application of a Multiplex Fluorescent PCR for Shigella Detection and Species Identification.Journal of fluorescence · 2022Article
- Development of high-resolution melting (HRM) assay to differentiate the species of Shigella isolates from stool and food samples.Scientific reports · 2022Article
- Article
- Development of multiplex cross displacement amplification combined with lateral flow biosensor assay for detection of virulentFrontiers in cellular and infection microbiology · 2022Article
- DNA sequencing, genomes and genetic markers of microbes on fruits and vegetables.Microbial biotechnology · 2021Review
- Molecular characterization of quinolone resistantIranian journal of microbiology · 2019Article
- Shifting national surveillance of Shigella infections toward geno-serotyping by the development of a tailored Luminex assay and NGS workflow.MicrobiologyOpen · 2019Article
- Utilization of Small RNA Genes to DistinguishThe American journal of tropical medicine and hygiene · 2019Article
- First investigation of the presence ofInfection and drug resistance · 2019Article
- An impedimetric aptasensor for Shigella dysenteriae using a gold nanoparticle-modified glassy carbon electrode.Mikrochimica acta · 2018Article
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4 authors.
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Abstract
The magnitude of shigellosis in developing countries is largely unknown because an affordable detection method is not available. Current laboratory diagnosis of Shigella spp. is laborious and time consuming and has low sensitivity. Hence, in the present study, a molecular-based diagnostic assay which amplifies simultaneously four specific genes to identify invC for Shigella genus, rfc for S. flexneri, wbgZ for S. sonnei, and rfpB for S. dysenteriae, as well as one internal control (ompA) gene, was developed in a single reaction to detect and differentiate Shigella spp. Validation with 120 Shigella strains and 37 non-Shigella strains yielded 100% specificity. The sensitivity of the PCR was 100 pg of genomic DNA, 5.4 × 10(4) CFU/ml, or approximately 120 CFU per reaction mixture of bacteria. The sensitivity of the pentaplex PCR assay was further improved following preincubation of the stool samples in gram-negative broth. A preliminary study with 30 diarrhoeal specimens resulted in no cross-reaction with other non-Shigella strains tested. We conclude that the developed pentaplex PCR assay is robust and can provide information about the four target genes that are essential for the identification of the Shigella genus and the three Shigella species responsible for the majority of shigellosis cases.
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