ArticleProceedings of the National Academy of Sciences of the United States of America2013
Synchronous activation of gonadotropin-releasing hormone gene transcription and secretion by pulsatile kisspeptin stimulation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 64 citations in OpenAlex.
- Novel KISS1 Gene Mutation Leading to Male Hypogonadotropic Hypogonadism.Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association · 2026Article
- Disruption of ovarian function and induction of apoptosis in female mice by Brefeldin A: Mechanistic insights into reproductive toxicity.Animal models and experimental medicine · 2025Article
- Critical Roles of the Circadian Transcription FactorFrontiers in endocrinology · 2022Review
- GnRH pulse frequency and irregularity play a role in male aging.Nature aging · 2021Article
- The transcription factors SIX3 and VAX1 are required for suprachiasmatic nucleus circadian output and fertility in female mice.Journal of neuroscience research · 2021Article
- Circadian Rhythms Within the Female HPG Axis: From Physiology to Etiology.Endocrinology · 2021Review
- Identifying signaling genes in spatial single-cell expression data.Bioinformatics (Oxford, England) · 2021Article
- Ultradian Rhythms in the Hypothalamic Arcuate Nucleus Kisspeptin Neurons and Developmental Processes.Molecules and cells · 2020Article
- Programming effects of maternal stress on the circadian system of adult offspring.Experimental & molecular medicine · 2020Article
- Role of core circadian clock genes in hormone release and target tissue sensitivity in the reproductive axis.Molecular and cellular endocrinology · 2020Review
- Kisspeptin Neuron-Specific and Self-Sustained Calcium Oscillation in the Hypothalamic Arcuate Nucleus of Neonatal Mice: Regulatory Factors of its Synchronization.Neuroendocrinology · 2020Article
- Circadian Function in Multiple Cell Types Is Necessary for Proper Timing of the Preovulatory LH Surge.Journal of biological rhythms · 2019Article
- The Contribution of the Circadian GeneJournal of the Endocrine Society · 2019Article
- Ultradian calcium rhythms in the paraventricular nucleus and subparaventricular zone in the hypothalamus.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Continuous Kisspeptin Administration in Postmenopausal Women: Impact of Estradiol on Luteinizing Hormone Secretion.The Journal of clinical endocrinology and metabolism · 2017Article
- Circadian Disruption Alters the Effects of Lipopolysaccharide Treatment on Circadian and Ultradian Locomotor Activity and Body Temperature Rhythms of Female Siberian Hamsters.Journal of biological rhythms · 2015Article
- Effect of Mefloquine, a Gap Junction Blocker, on Circadian Period2 Gene Oscillation in the Mouse Suprachiasmatic Nucleus Ex Vivo.Endocrinology and metabolism (Seoul, Korea) · 2015Article
- Gonadotropin and kisspeptin gene expression, but not GnRH, are impaired in cFOS deficient mice.Molecular and cellular endocrinology · 2015Article
- Circadian Tick-Talking Across the Neuroendocrine System and Suprachiasmatic Nuclei Circuits: The Enigmatic Communication Between the Molecular and Electrical Membrane Clocks.Journal of neuroendocrinology · 2015Review
- A Multi-Oscillatory Circadian System Times Female Reproduction.Frontiers in endocrinology · 2015Review
Corrections and comments
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Authors and funding
9 authors at 7 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pulsatile release of hypothalamic gonadotropin-releasing hormone (GnRH) is essential for pituitary gonadotrope function. Although the importance of pulsatile GnRH secretion has been recognized for several decades, the mechanisms underlying GnRH pulse generation in hypothalamic neural networks remain elusive. Here, we demonstrate the ultradian rhythm of GnRH gene transcription in single GnRH neurons using cultured hypothalamic slices prepared from transgenic mice expressing a GnRH promoter-driven destabilized luciferase reporter. Although GnRH promoter activity in each GnRH neuron exhibited an ultradian pattern of oscillations with a period of ∼10 h, GnRH neuronal cultures exhibited partially synchronized bursts of GnRH transcriptional activity at ∼2-h intervals. Surprisingly, pulsatile administration of kisspeptin, a potent GnRH secretagogue, evoked dramatic synchronous activation of GnRH gene transcription with robust stimulation of pulsatile GnRH secretion. We also addressed the issue of hierarchical interaction between the circadian and ultradian rhythms by using Bmal1-deficient mice with defective circadian clocks. The circadian molecular oscillator barely affected basal ultradian oscillation of GnRH transcription but was heavily involved in kisspeptin-evoked responses of GnRH neurons. In conclusion, we have clearly shown synchronous bursts of GnRH gene transcription in the hypothalamic GnRH neuronal population in association with episodic neurohormone secretion, thereby providing insight into GnRH pulse generation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.