ArticleInfection and immunity2013
Human primary intestinal epithelial cells as an improved in vitro model for Cryptosporidium parvum infection.
Article in Infection and immunity, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 71 citations in OpenAlex.
- Two- and Three-Dimensional Human Intestinal Tissue Models for Cryptosporidium Infection.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Gut Barrier Dysfunction and Microbiota Variations in Cryptosporidiosis: A Comprehensive Review.Veterinary sciences · 2025Review
- Anti-Cryptosporidial Drug-Discovery Challenges and Existing Therapeutic Avenues: A "One-Health" Concern.Life (Basel, Switzerland) · 2024Review
- Differential Response to the Course of Cryptosporidium parvum Infection and Its Impact on Epithelial Integrity in Differentiated versus Undifferentiated Human Intestinal Enteroids.Infection and immunity · 2022Article
- Fecal luminal factors from patients with irritable bowel syndrome induce distinct gene expression of colonoids.Neurogastroenterology and motility · 2022Article
- The Antioxidant Effect of Natural Antimicrobials in Shrimp Primary Intestinal Cells Infected withAntioxidants (Basel, Switzerland) · 2022Article
- Article
- Breakthroughs in microbiology made possible with organoids.PLoS pathogens · 2021Article
- First Metabolic Insights intoBiology · 2021Article
- Review
- Organoids: a promising new in vitro platform in livestock and veterinary research.Veterinary research · 2021Review
- The Stem Cell Revolution Revealing Protozoan Parasites' Secrets and Paving the Way towards Vaccine Development.Vaccines · 2021Review
- The Mucosal Innate Immune Response toFrontiers in cellular and infection microbiology · 2021Review
- Intestinal organoid/enteroid-based models for Cryptosporidium.Current opinion in microbiology · 2020Review
- Organoids and Bioengineered Intestinal Models: Potential Solutions to theMicroorganisms · 2020Review
- Robotically handled whole-tissue culture system for the screening of oral drug formulations.Nature biomedical engineering · 2020Article
- Two- and Three-Dimensional Bioengineered Human Intestinal Tissue Models for Cryptosporidium.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Generation of human liver organoids from pluripotent stem cell-derived hepatic endoderms.PeerJ · 2020Article
- (10Molecules (Basel, Switzerland) · 2019Article
- Comparative Pathobiology of the Intestinal Protozoan ParasitesPathogens (Basel, Switzerland) · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The study of human intestinal pathogens has been limited by the lack of methods for the long-term culture of primary human intestinal epithelial cells (PECs). The development of infection models with PECs would allow a better understanding of host-parasite interactions. The objective of this study was to develop a novel method for prolonged in vitro cultivation of PECs that can be used to study Cryptosporidium infection. We isolated intact crypts from human intestines removed during weight loss surgery. The fragments of intestinal layers were cultivated with culture medium supplemented with growth factors and antiapoptotic molecules. After 7 days, the PECs formed self-regenerating cell clusters, forming villi that resemble intestinal epithelium. The PECs proliferated and remained viable for at least 60 days. The cells expressed markers for intestinal stem cells, epithelial cells, and mature enterocytes. The PECs were infected with Cryptosporidium. In contrast to older models in which parasite numbers decay, the burden of parasites increased for >120 h. In summary, we describe here a novel method for the cultivation of self-regenerating human epithelial cells from small intestinal crypts, which contain both intestinal stem cells and mature villus cells. We present data that suggest these cells support Cryptosporidium better than existing cell lines. PECs should provide an improved tool for studying host-parasite interactions involving Cryptosporidium and other intestinal pathogens.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.