ArticleInfection and immunity2013
The fimbrial protein FlfA from Gallibacterium anatis is a virulence factor and vaccine candidate.
Article in Infection and immunity, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 49 citations in OpenAlex.
- In silico prediction of Gallibacterium anatis pan-immunogens.Veterinary research · 2014Trial
- Development and Validation of an In-House Indirect ELISA for Serological Detection ofVeterinary medicine international · 2026Article
- Article
- Comparative genomics andFrontiers in microbiology · 2026Article
- Metagenomic analysis and proteins prediction of emerging pathogens in artisanal cheese.Molecular diversity · 2025Article
- Genomic characteristics, antimicrobial resistance profiles and virulence factors of Gallibacterium anatis isolates from layer chickens in Northern China.BMC microbiology · 2025Article
- Multidrug Resistance ofPathogens (Basel, Switzerland) · 2024Article
- Antibiotic resistance ofJournal of veterinary research · 2024Article
- Gallibacterium anatis infection in poultry: a comprehensive review.Tropical animal health and production · 2023Review
- Isolation and characterization of multidrug resistant Gallibacterium anatis biovar haemolytica strains from Polish geese and hens.Veterinary research · 2023Article
- A Maverick-like cluster in the genome of a pathogenic, moderately virulent strain ofFrontiers in microbiology · 2023Article
- Sequence Analysis, Antibiogram Profile, Virulence and Antibiotic Resistance Genes of XDR and MDRInfection and drug resistance · 2022Article
- A putative siderophore receptor ofFrontiers in microbiology · 2022Article
- Co-Infection ofPathogens (Basel, Switzerland) · 2021Article
- Clonal spread of multi-resistant Gallibacterium anatis isolates among Iranian broilers and layers.Veterinary research · 2021Article
- Antibacterial Activity of Lactic Acid ProducingFrontiers in veterinary science · 2021Article
- Etiology, epidemiology, pathology, and advances in diagnosis, vaccine development, and treatment ofThe veterinary quarterly · 2020Review
- Immune Suppression Induced byToxins · 2020Article
- GtxA is a virulence factor that promotes a Th2-like response during Gallibacterium anatis infection in laying hens.Veterinary research · 2020Article
- Hydrostatic Filtration Enables Large-Scale Production of Outer Membrane Vesicles That Effectively Protect Chickens againstVaccines · 2020Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Gram-negative bacterium Gallibacterium anatis is a major cause of salpingitis and peritonitis in egg-laying chickens, leading to decreased egg production worldwide. Widespread multidrug resistance largely prevents treatment of this organism using traditional antimicrobial agents, while antigenic diversity hampers disease prevention by classical vaccines. Thus, insight into its pathogenesis and knowledge about important virulence factors is urgently required. A key event during the colonization and invasion of mucosal surfaces is adherence, and recently, at least three F17-like fimbrial gene clusters were identified in the genomes of several G. anatis strains. The objective of this study was to characterize the putative F17-like fimbrial subunit protein FlfA from G. anatis 12656-12 and determine its importance for virulence. In vitro expression and surface exposure of FlfA was demonstrated by flow cytometry and immunofluorescence microscopy. The predicted function of FlfA as a fimbrial subunit protein was confirmed by immunogold electron microscopy. An flfA deletion mutant (ΔflfA) was generated in G. anatis 12656-12, and importantly, this mutant was significantly attenuated in the natural chicken host. Furthermore, protection against G. anatis 12656-12 could be induced by immunizing chickens with recombinant FlfA. Finally, in vitro expression of FlfA homologs was observed in a genetically diverse set of G. anatis strains, suggesting the potential of FlfA as a serotype-independent vaccine candidate This is the first study describing a fimbrial subunit protein of G. anatis with a clear potential as a vaccine antigen.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.