ArticleThe American journal of tropical medicine and hygiene2013
A novel, multi-parallel, real-time polymerase chain reaction approach for eight gastrointestinal parasites provides improved diagnostic capabilities to resource-limited at-risk populations.
Article in The American journal of tropical medicine and hygiene, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 166 papers, 1 of them a synthesis that pooled it.
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Who cites it
166 citing papers in PubMed, 1 synthesis or guideline pooled it, 271 citations in OpenAlex.
- Current epidemiological evidence for predisposition to high or low intensity human helminth infection: a systematic review.Parasites & vectors · 2018Pooled it
- Determination of diagnostic cycle threshold cutoffs for qPCR-based prevalence surveys of soil-transmitted helminth infections.Microbiology spectrum · 2026Trial
- Impact of Anthelmintic Therapy for Invasive Helminth Infection on Microbial Translocation, Inflammation, and Immune Response among Ugandans Living with HIV: A Randomized Proof of Concept Study.The American journal of tropical medicine and hygiene · 2025Trial
- Performance of real-time polymerase chain reaction and Kato-Katz for diagnosing soil-transmitted helminth infections and evaluating treatment efficacy of emodepside in randomized controlled trials.PLoS neglected tropical diseases · 2025Trial
- A comparison of the diagnostic capability of Kato-Katz and real-time PCR for the assessment of treatment efficacy of ivermectin and albendazole combination against T. trichiura infections.PLoS neglected tropical diseases · 2024Trial
- Egg excretion patterns of soil-transmitted helminth infections in humans following albendazole-ivermectin and albendazole treatment.PLoS neglected tropical diseases · 2024Trial
- Performance of the Kato-Katz method and real time polymerase chain reaction for the diagnosis of soil-transmitted helminthiasis in the framework of a randomised controlled trial: treatment efficacy and day-to-day variation.Parasites & vectors · 2020Trial
- Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAKG2 and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries.PLoS neglected tropical diseases · 2019Trial
- Effects of water, sanitation, handwashing and nutritional interventions on soil-transmitted helminth infections in young children: A cluster-randomized controlled trial in rural Bangladesh.PLoS neglected tropical diseases · 2019Trial
- Development of a multiplex quantitative real-time PCR (qPCR) assay for simultaneous detection of Schistosoma haematobium and high-risk human papillomaviruses.PLoS neglected tropical diseases · 2026Article
- Chronic exposure to intestinal parasites and bacterial enteropathogens among children in rural Madagascar: Implications for asymptomatic carriage and co-infections.PLoS neglected tropical diseases · 2026Article
- Helminth-infected Mozambican children with malaria have increased anaemia, cytokines and helminth-specific antibodies.PLoS neglected tropical diseases · 2026Article
- Development of Recombinase Polymerase Amplification and CRISPR-Cas12a-Enhanced Isothermal Amplification Assays for Strongyloides stercoralis DNA Detection: A Pilot Study.The American journal of tropical medicine and hygiene · 2026Article
- Prevalence of multiple human intestinal parasites across diverse environments in Madagascar.PLoS neglected tropical diseases · 2026Article
- Assessment of water quality and microbial contamination in Santa Marta's major rivers using conventional methods and next-generation sequencing.Environmental science and pollution research international · 2026Article
- Helminth coinfections mitigate clinical, parasitological, and immune outcomes in Mozambican children with malaria.BMC medicine · 2026Article
- Bacterial fecal microbiome composition associated with HIV stage among rural and peri-urban adults living with HIV in Uganda.BMC infectious diseases · 2026Article
- Molecular surveillance of intestinal parasites and Acanthamoeba species in soils from outdoor built environments in rural northwestern Argentina.Parasite (Paris, France) · 2026Article
- Evaluation of Each ThreeMicroorganisms · 2025Article
- Harnessing Molecular Techniques to Inform Species-Specific Hookworm Prevalence and Infection Intensity and Detect Strongyloides in Dak Lak Province, Vietnam.The American journal of tropical medicine and hygiene · 2025Article
106 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Diagnosis of gastrointestinal parasites has traditionally relied on stool microscopy, which has low diagnostic sensitivity and specificity. We have developed a novel, rapid, high-throughput quantitative multi-parallel real-time polymerase chain reaction (qPCR) platform. Species-specific primers/probes were used for eight common gastrointestinal parasite pathogens: Ascaris lumbricoides, Necator americanus, Ancylostoma duodenale, Giardia lamblia, Cryptosporidium spp., Entamoeba histolytica, Trichuris trichiura, and Strongyloides stercoralis. Stool samples from 400 13-month-old children in rural Ecuador were analyzed and the qPCR was compared with a standard direct wet mount slide for stool microscopy, as were 125 8-14-year-old children before and after anthelmintic treatment. The qPCR showed higher detection rates for all parasites compared with direct microscopy, Ascaris (7.0% versus 5.5%) and for Giardia (31.5% versus 5.8%). Using an enhanced DNA extraction method, we were able to detect T. trichiura DNA. These assays will be useful to refine treatment options for affected populations, ultimately leading to better health outcomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.