Evidence map›Paper›PMID 23507273›Full record

ArticleAntimicrobial agents and chemotherapy2013

Simple resazurin-based microplate assay for measuring Chlamydia infections.

Ichie Osaka, P Scott Hefty

Open access · bronzeAbstract readEvaluation Study
In one paragraph

Article in Antimicrobial agents and chemotherapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 32 citations in OpenAlex.

  1. Article
  2. Article
  3. HybridPharmaceuticals (Basel, Switzerland) · 2025
    Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Red FluorescentFrontiers in microbiology · 2018
    Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Ichie OsakaDepartment of Molecular Biosciences, University of Kansas, Lawrence, Kansas, USA.
P Scott Hefty
University of Kansas · US

Funding

Biomechanical and Computational Molecular Design of Microbicide Delivery SystemsR33AI082697 · NIAID · UNIVERSITY OF KANSAS LAWRENCE · PI KIEWEG, SARAH · 2011 to 2013
$1.3M
Biomechanical and Computational Molecular Design of Microbicide Delivery SystemsR21AI082697 · NIAID · UNIVERSITY OF KANSAS LAWRENCE · PI KIEWEG, SARAH · 2009 to 2010
$400k
NIAID NIH HHS NIH AI082697NIAID NIH HHS R21 AI082697NIAID NIH HHS R33 AI082697
6 · The paper itself

Abstract

The conventional method for quantification of Chlamydia infection using fluorescence microscopy typically involves time- and labor-intensive manual enumeration, which is not applicable for a large-scale analysis required for an inhibitory compound screen. In this study, an alamarBlue (resazurin) assay was adopted to measure Chlamydia infection by measuring the redox capability of infected host cells in a 96-well format. The assay provided measurements comparable to those of the conventional microscopy method while drastically reducing the time required for analysis.

Indexed as

AnimalsAnti-Bacterial AgentsBiological AssayCell SurvivalChlamydia InfectionsChlamydia trachomatisFibroblastsHumansIndicators and ReagentsL CellsMiceMicrobial Sensitivity TestsOxazinesPolymyxin BTetracyclineXanthenesAnti-Bacterial AgentsIndicators and ReagentsOxazinesPolymyxin BresazurinTetracyclineXanthenes

Identifiers

PMID23507273
PMCPMC3716173
OpenAlexW1981681959

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.