ArticleExperimental neurology2013
Sox10-MCS5 enhancer dynamically tracks human oligodendrocyte progenitor fate.
Article in Experimental neurology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Transcription factor induction of human oligodendrocyte progenitor fate and differentiation.Proceedings of the National Academy of Sciences of the United States of America · 2014Trial
- A pipeline for cell migration analysis in live-cell imaging data from human iPSC-derived forebrain assembloids.Frontiers in cell and developmental biology · 2026Article
- Developing a minimally invasive gene therapy for multiple sclerosis.Molecular therapy. Methods & clinical development · 2025Article
- Injectable shear-thinning hydrogels promote oligodendrocyte progenitor cell survival and remyelination in the central nervous system.Science advances · 2024Article
- Oscillatory calcium release and sustained store-operated oscillatory calcium signaling prevents differentiation of human oligodendrocyte progenitor cells.Scientific reports · 2022Article
- Overcoming the inhibitory microenvironment surrounding oligodendrocyte progenitor cells following experimental demyelination.Nature communications · 2021Article
- Evolutionary Origins of the Oligodendrocyte Cell Type and Adaptive Myelination.Frontiers in neuroscience · 2021Review
- Oligodendrocyte Intrinsic miR-27a Controls Myelination and Remyelination.Cell reports · 2019Article
- Paired Related Homeobox Protein 1 Regulates Quiescence in Human Oligodendrocyte Progenitors.Cell reports · 2018Article
- Muscarinic Receptor MThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- SOX10 Single Transcription Factor-Based Fast and Efficient Generation of Oligodendrocytes from Human Pluripotent Stem Cells.Stem cell reports · 2018Article
- Targeting human oligodendrocyte progenitors for myelin repair.Experimental neurology · 2016Review
- Sox10 Expression in Goldfish Retina and Optic Nerve Head in Controls and after the Application of Two Different Lesion Paradigms.PloS one · 2016Article
- CD133/CD140a-based isolation of distinct human multipotent neural progenitor cells and oligodendrocyte progenitor cells.Stem cells and development · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
In this study, we sought to establish a novel method to prospectively and dynamically identify live human oligodendrocyte precursor cells (OPCs) and oligodendrocyte lineage cells from brain dissociates and pluripotent stem cell culture. We selected a highly conserved enhancer element of the Sox10 gene, known as MCS5, which directs reporter expression to oligodendrocyte lineage cells in mouse and zebrafish. We demonstrate that lentiviral Sox10-MCS5 induced expression of GFP at high levels in a subpopulation of human CD140a/PDGFαR-sorted OPCs as well as their immature oligodendrocyte progeny. Furthermore, we show that almost all Sox10-MCS5:GFP(high) cells expressed OPC antigen CD140a and human OPCs expressing SOX10, OLIG2, and PDGFRA mRNAs could be prospectively identified using GFP based fluorescence activated cells sorting alone. Additionally, we established a human induced pluripotent cell (iPSC) line transduced with the Sox10-MCS5:GFP reporter using a Rex-Neo cassette. Similar to human primary cells, GFP expression was restricted to embryoid bodies containing both oligodendrocyte progenitor and oligodendrocyte cells and co-localized with NG2 and O4-positive cells respectively. As such, we have developed a novel reporter system that can track oligodendrocyte commitment in human cells, establishing a valuable tool to improve our understanding and efficiency of human oligodendrocyte derivation.
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Registered trials
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