ArticlePloS one2013
Characterization and functional analysis of four glutathione S-transferases from the migratory locust, Locusta migratoria.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 82 citations in OpenAlex.
- Sigma-Class Glutathione Transferases (GSTσ): A New Target with Potential for Helminth Control.Tropical medicine and infectious disease · 2024Review
- Antennal transcriptomic analysis of carboxylesterases and glutathione S-transferases associated with odorant degradation in the tea gray geometrid,Frontiers in physiology · 2023Article
- A chromosome-level genome assembly provides insights into the environmental adaptability and outbreaks of Chlorops oryzae.Communications biology · 2022Article
- Response of xenobiotic biodegradation and metabolic genes in Tribolium castaneum following eugenol exposure.Molecular genetics and genomics : MGG · 2022Article
- Gut transcriptome of two bark beetle species stimulated with the same kairomones reveals molecular differences in detoxification pathways.Computational and structural biotechnology journal · 2022Article
- Structural and Functional Characterization of One Unclassified Glutathione S-Transferase in Xenobiotic Adaptation ofInternational journal of molecular sciences · 2021Article
- Potentiation Effects of Ficus sycomorus Active Fraction Against Permethrin-Resistant Field-Population of Anopheles coluzzii (Diptera: Culicidae).Neotropical entomology · 2021Article
- Article
- Olive Leaf Extracts Toxicity to the Migratory Locust, Locusta migratoria: Histopathological Effects on the Alimentary Canal and Acetylcholinesterase and Glutathione S-Transferases Activity.Neotropical entomology · 2019Article
- Article
- Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead.Environmental science and pollution research international · 2019Article
- Glutathione S-transferases play a role in the detoxification of flumethrin and chlorpyrifos in Haemaphysalis longicornis.Parasites & vectors · 2018Article
- Multiple Glutathione S-Transferase Genes in Heortia vitessoides (Lepidoptera: Crambidae): Identification and Expression Patterns.Journal of insect science (Online) · 2018Article
- Enzymatic activity induction of GST-family isoenzymes from pesticide mixture used in floriculture.Environmental science and pollution research international · 2018Article
- De novo transcriptomic analysis of the alimentary tract of the tephritid gall fly, Procecidochares utilis.PloS one · 2018Article
- Antioxidant Role ofFrontiers in physiology · 2018Article
- Glutathione-S-Transferases in the Olfactory Organ of the Noctuid MothFrontiers in physiology · 2018Article
- A sublethal dose of a neonicotinoid insecticide disrupts visual processing and collision avoidance behaviour in Locusta migratoria.Scientific reports · 2017Article
- The Potential Coordination of the Heat-Shock Proteins and Antioxidant Enzyme Genes ofFrontiers in physiology · 2017Article
- SeGSTo, a novel glutathione S-transferase from the beet armyworm (Spodoptera exigua), involved in detoxification and oxidative stress.Cell stress & chaperones · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glutathione S-transferases (GSTs) play an important role in detoxification of xenobiotics in both prokaryotic and eukaryotic cells. In this study, four GSTs (LmGSTd1, LmGSTs5, LmGSTt1, and LmGSTu1) representing different classes were identified from the migratory locust, Locusta migratoria. These four proteins were heterologously expressed in Escherichia coli as soluble fusion proteins, purified by Ni(2+)-nitrilotriacetic acid agarose column and biochemically characterized. LmGSTd1, LmGSTs5, and LmGSTu1 showed high activities with 1-chloro-2, 4-dinitrobenzene (CDNB), detectable activity with p-nitro-benzyl chloride (p-NBC) and 1, 2-dichloro-4-nitrobenzene (DCNB), whereas LmGSTt1 showed high activity with p-NBC and detectable activity with CDNB. The optimal pH of the locust GSTs ranged between 7.0 to 9.0. Ethacrynic acid and reactive blue effectively inhibited all four GSTs. LmGSTs5 was most sensitive to heavy metals (Cu(2+) and Cd(2+)). The maximum expression of the four GSTs was observed in Malpighian tubules and fat bodies as evaluated by western blot. The nymph mortalities after carbaryl treatment increased by 28 and 12% after LmGSTs5 and LmGSTu1 were silenced, respectively. The nymph mortalities after malathion and chlorpyrifos treatments increased by 26 and 18% after LmGSTs5 and LmGSTu1 were silenced, respectively. These results suggest that sigma GSTs in L. migratoria play a significant role in carbaryl detoxification, whereas some of other GSTs may also involve in the detoxification of carbaryl and chlorpyrifos.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.