ArticlePLoS genetics2013
A WRKY transcription factor recruits the SYG1-like protein SHB1 to activate gene expression and seed cavity enlargement.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 76 citations in OpenAlex.
- The IKU2/RLK7-MAPK-MINI3 signaling module controls endosperm cellularization timing to determine seed size in Arabidopsis.Journal of integrative plant biology · 2026Article
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- The PHO1 protein family in phosphate export and beyond.Plant physiology · 2026Review
- Removal of seed coat H3K27me3 in Arabidopsis requires noncanonical functions of brassinosteroid receptors.The Plant cell · 2026Article
- Genome-wide identification and functional analysis of the WRKY transcription factor family in Reynoutria japonica reveals its role in balancing growth and abiotic stress tolerance.BMC plant biology · 2026Article
- Anatomical and Transcriptomic Analyses Revealed the Key Genes Associated with Tuber Expansion inGenes · 2026Article
- Article
- When Size Matters: New Insights on How Seed Size Can Contribute to the Early Stages of Plant Development.Plants (Basel, Switzerland) · 2024Review
- Understanding the Molecular Regulatory Networks of Seed Size in Soybean.International journal of molecular sciences · 2024Review
- Transcriptome analysis of ovules offers early developmental clues after fertilization in3 Biotech · 2023Article
- Photoperiod controls plant seed size in a CONSTANS-dependent manner.Nature plants · 2023Article
- Developing Genetic Engineering Techniques for Control of Seed Size and Yield.International journal of molecular sciences · 2022Review
- WRKY2 and WRKY10 regulate the circadian expression ofPlant communications · 2022Article
- Transcriptome and metabolome analyses reveal the key genes related to grain size of big grain mutant in Tartary Buckwheat (Frontiers in plant science · 2022Article
- Identification of miRNAs-mediated seed and stone-hardening regulatory networks and their signal pathway of GA-induced seedless berries in grapevine (V. vinifera L.).BMC plant biology · 2021Article
- The Arabidopsis MATERNAL EFFECT EMBRYO ARREST45 protein modulates maternal auxin biosynthesis and controls seed size by inducing AINTEGUMENTA.The Plant cell · 2021Article
- Cross Inhibition of MPK10 and WRKY10 Participating in the Growth of Endosperm inFrontiers in plant science · 2021Article
- Article
- Mobile TERMINAL FLOWER1 determines seed size in Arabidopsis.Nature plants · 2020Article
- Into the Seed: Auxin Controls Seed Development and Grain Yield.International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Seed development in Arabidopsis and in many dicots involves an early proliferation of the endosperm to form a large embryo sac or seed cavity close to the size of the mature seed, followed by a second phase during which the embryo grows and replaces the endosperm. Short hypocotyl under BLUE1 (SHB1) is a member of the SYG1 protein family in fungi, Caenorhabditis elegans, flies, and mammals. SHB1 gain-of-function enhances endosperm proliferation, increases seed size, and up-regulates the expression of the WRKY transcription factor gene MINISEED3 (MINI3) and the LRR receptor kinase gene HAIKU2 (IKU2). Mutations in either IKU2 or MINI3 retard endosperm proliferation and reduce seed size. However, the molecular mechanisms underlying the establishment of the seed cavity and hence the seed size remain largely unknown. Here, we show that the expression of MINI3 and IKU2 is repressed before fertilization and after 4 days after pollination (DAP), but is activated by SHB1 from 2 to 4 DAP prior to the formation of the seed cavity. SHB1 associates with their promoters but without a recognizable DNA binding motif, and this association is abolished in mini3 mutant. MINI3 binds to W-boxes in, and recruits SHB1 to, its own and IKU2 promoters. Interestingly, SHB1, but not MINI3, activates transcription of pMINI3::GUS or pIKU2::GUS. We reveal a critical developmental switch through the activation of MINI3 expression by SHB1. The recruitment of SHB1 by MINI3 to its own and IKU2 promoters represents a novel two-step amplification to counter the low expression level of IKU2, which is a trigger for endosperm proliferation and seed cavity enlargement.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.