ArticlePLoS computational biology2013
Positively selected sites in cetacean myoglobins contribute to protein stability.
Article in PLoS computational biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 63 citations in OpenAlex.
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- Population structure and mitogenomic analyses reveal dispersal routes of Macrobrachium nipponense in China.BMC genomics · 2025Article
- Rapid evolutionary change in trait correlations of single proteins.Nature communications · 2024Article
- Molecular Footprints on Osmoregulation-Related Genes Associated with Freshwater Colonization by Cetaceans and Sirenians.Journal of molecular evolution · 2023Article
- Stability and expression of SARS-CoV-2 spike-protein mutations.Molecular and cellular biochemistry · 2023Article
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- Genetics of adaptation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Genomic insights into the secondary aquatic transition of penguins.Nature communications · 2022Article
- Intermolecular Interactions Drive Protein Adaptive and Coadaptive Evolution at Both Species and Population Levels.Molecular biology and evolution · 2022Article
- Common and unique strategies of myoglobin evolution for deep-sea adaptation of diving mammals.iScience · 2021Article
- Tracing whale myoglobin evolution by resurrecting ancient proteins.Scientific reports · 2018Article
- Sequence-Based Analysis of Thermal Adaptation and Protein Energy Landscapes in an Invasive Blue Mussel (Mytilus galloprovincialis).Genome biology and evolution · 2017Article
- Superoxide dismutase 1 is positively selected to minimize protein aggregation in great apes.Cellular and molecular life sciences : CMLS · 2017Article
- Bridging the physical scales in evolutionary biology: from protein sequence space to fitness of organisms and populations.Current opinion in structural biology · 2017Review
- Positive Selection Linked with Generation of Novel Mammalian Dentition Patterns.Genome biology and evolution · 2016Article
- The thermostability and specificity of ancient proteins.Current opinion in structural biology · 2016Review
- Evolutionary Genetics of Hypoxia Tolerance in Cetaceans during Diving.Genome biology and evolution · 2016Article
- The acquisition of novel N-glycosylation sites in conserved proteins during human evolution.BMC bioinformatics · 2015Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Since divergence ∼50 Ma ago from their terrestrial ancestors, cetaceans underwent a series of adaptations such as a ∼10-20 fold increase in myoglobin (Mb) concentration in skeletal muscle, critical for increasing oxygen storage capacity and prolonging dive time. Whereas the O2-binding affinity of Mbs is not significantly different among mammals (with typical oxygenation constants of ∼0.8-1.2 µM(-1)), folding stabilities of cetacean Mbs are ∼2-4 kcal/mol higher than for terrestrial Mbs. Using ancestral sequence reconstruction, maximum likelihood and bayesian tests to describe the evolution of cetacean Mbs, and experimentally calibrated computation of stability effects of mutations, we observe accelerated evolution in cetaceans and identify seven positively selected sites in Mb. Overall, these sites contribute to Mb stabilization with a conditional probability of 0.8. We observe a correlation between Mb folding stability and protein abundance, suggesting that a selection pressure for stability acts proportionally to higher expression. We also identify a major divergence event leading to the common ancestor of whales, during which major stabilization occurred. Most of the positively selected sites that occur later act against other destabilizing mutations to maintain stability across the clade, except for the shallow divers, where late stability relaxation occurs, probably due to the shorter aerobic dive limits of these species. The three main positively selected sites 66, 5, and 35 undergo changes that favor hydrophobic folding, structural integrity, and intra-helical hydrogen bonds.
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