ArticleJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research2013
Tracking circadian rhythms of bone mineral deposition in murine calvarial organ cultures.
Article in Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed, 64 citations in OpenAlex.
- Modeling the Clockwork of Bone: A Narrative Review of Experimental Approaches to Circadian Rhythm in Bone Metabolism.International journal of molecular sciences · 2026Review
- Neural regulation of bone: from central neural circuits to peripheral innervation of the skeletal stem cell niche.Bone research · 2026Review
- Chronobiology of fracture repair: local molecular clocks, rhythm protection, and time-anchored translation.Frontiers in endocrinology · 2026Review
- Chronotherapy and intervertebral disc degeneration: understanding the role of circadian rhythm in degenerative processes.Frontiers in cell and developmental biology · 2025Review
- Skeletal Phenotyping of Period-1-Deficient Melatonin-Proficient Mice.Journal of pineal research · 2024Article
- Bone hierarchical organization through the lens of materials science: Present opportunities and future challenges.Bone reports · 2024Review
- The specificities, influencing factors, and medical implications of bone circadian rhythms.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Review
- Circadian rhythm disruption upregulating Per1 in mandibular condylar chondrocytes mediating temporomandibular joint osteoarthritis via GSK3β/β-CATENIN pathway.Journal of translational medicine · 2024Article
- Combining Raman Microspectroscopy and X-ray Microcomputed Tomography for the Study of Bone Quality in Apolipoprotein-Deficient Animal Models.Molecules (Basel, Switzerland) · 2023Article
- Effect of Circadian Rhythm Disturbance on the Human Musculoskeletal System and the Importance of Nutritional Strategies.Nutrients · 2023Review
- Multimodal and Multiscale Characterization of the Bone-Bacteria Interface in a Case of Medication-Related Osteonecrosis of the Jaw.JBMR plus · 2022Article
- Low intensity near-infrared light promotes bone regeneration via circadian clock protein cryptochrome 1.International journal of oral science · 2022Article
- Rhythm disturbance in osteoarthritis.Cell communication and signaling : CCS · 2022Review
- The role of circadian rhythm in osteoporosis; a review.Frontiers in cell and developmental biology · 2022Review
- Spatial bias in cAMP generation determines biological responses to PTH type 1 receptor activation.Science signaling · 2021Article
- Circadian rhythms affect bone reconstruction by regulating bone energy metabolism.Journal of translational medicine · 2021Review
- Evaluation of the optimal dosage of BMP-9 through the comparison of bone regeneration induced by BMP-9 versus BMP-2 using an injectable microparticle embedded thermosensitive polymeric carrier in a rat cranial defect model.Materials science & engineering. C, Materials for biological applications · 2021Article
- Article
- Understanding Bone Disease in Patients with Diabetic Kidney Disease: a Narrative Review.Current osteoporosis reports · 2020Review
- Towards refining Raman spectroscopy-based assessment of bone composition.Scientific reports · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Osteoblasts, which orchestrate the deposition of small apatite crystals through the expression of nucleating proteins, have been shown to also express clock genes associated with the circadian signaling pathway. We hypothesized that protein-mediated bone mineralization may be linked to circadian oscillator mechanisms functioning in peripheral bone tissue. In this study, Per1 expression in ex vivo neonatal murine calvaria organ cultures was monitored for 6 days using a Per1-luciferase transgene as a bioluminescent indicator of clock function. Fluctuations in Per1 expression had a period of 25 ± 4 hours (n = 14) with early expression at CT09:59 ± 03:37 (CT = circadian time). We also established the kinetics of mineral deposition in developing bone by using noninvasive Raman microscopy to track mineral accumulation in calvarial tissue. The content and quality of newly deposited mineral was continually examined at the interparietal bone/fontanel boundary for a period of 6 days with 1-hour temporal resolution. Using this approach, mineralization over time exhibited bursts of mineral deposition followed by little or no deposition, which was recurrent with a periodicity of 26.8 ± 9.6 hours. As many as six near-daily mineralization events were observed in the calvaria before deposition ceased. Earliest mineralization events occurred at CT16:51 ± 03:45, which is 6 hours behind Per1 expression. These findings are consistent with the hypothesis that mineralization in developing bone tissue is regulated by a local circadian oscillator mechanism.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.