ArticleJournal of bacteriology2013
Characterization of undermethylated sites in Vibrio cholerae.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 53 citations in OpenAlex.
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- The molecular mechanism for carbon catabolite repression of the chitin response in Vibrio cholerae.PLoS genetics · 2023Article
- The PilT retraction ATPase promotes both extension and retraction of the MSHA type IVa pilus in Vibrio cholerae.PLoS genetics · 2022Article
- Deficiency in cytosine DNA methylation leads to high chaperonin expression and tolerance to aminoglycosides in Vibrio cholerae.PLoS genetics · 2021Article
- Genomic Characterization of a Prophage, Smhb1, That InfectsMicroorganisms · 2021Article
- Fresh Extension of Vibrio cholerae Competence Type IV Pili Predisposes Them for Motor-Independent Retraction.Applied and environmental microbiology · 2021Article
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- Multiplexed Competition in a Synthetic Squid Light Organ Microbiome Using Barcode-Tagged Gene Deletions.mSystems · 2020Article
- Contribution of DNA adenine methylation to gene expression heterogeneity in Salmonella enterica.Nucleic acids research · 2020Article
- Species-Specific Quorum Sensing Represses the Chitobiose Utilization Locus in Vibrio cholerae.Applied and environmental microbiology · 2020Article
- Experimental approaches to tracking mobile genetic elements in microbial communities.FEMS microbiology reviews · 2020Review
- ChiS is a noncanonical DNA-binding hybrid sensor kinase that directly regulates the chitin utilization program inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Antibiotic Resistance and Epigenetics: More to It than Meets the Eye.Antimicrobial agents and chemotherapy · 2020Review
- PilT and PilU are homohexameric ATPases that coordinate to retract type IVa pili.PLoS genetics · 2019Article
- The master quorum-sensing regulators LuxR/HapR directly interact with the alpha subunit of RNA polymerase to drive transcription activation in Vibrio harveyi and Vibrio cholerae.Molecular microbiology · 2019Article
- Deciphering bacterial epigenomes using modern sequencing technologies.Nature reviews. Genetics · 2019Review
- ComM is a hexameric helicase that promotes branch migration during natural transformation in diverse Gram-negative species.Nucleic acids research · 2018Article
- Systematic genetic dissection of chitin degradation and uptake in Vibrio cholerae.Environmental microbiology · 2017Article
- Multiplex Genome Editing by Natural Transformation (MuGENT) for Synthetic Biology in Vibrio natriegens.ACS synthetic biology · 2017Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The activities of DNA methyltransferases are important for a variety of cellular functions in bacteria. In this study, we developed a modified high-throughput technique called methyl homopolymer tail mediated sequencing (methyl HTM-seq) to identify the undermethylated sites in the Vibrio cholerae genome for the two DNA methyltransferases, Dam, an adenine methyltransferase, and VchM, a cytosine methyltransferase, during growth in rich medium in vitro. Many of the undermethylated sites occurred in intergenic regions, and for most of these sites, we identified the transcription factors responsible for undermethylation. This confirmed the presence of previously hypothesized DNA-protein interactions for these transcription factors and provided insight into the biological state of these cells during growth in vitro. DNA adenine methylation has previously been shown to mediate heritable epigenetic switches in gene regulation. However, none of the undermethylated Dam sites tested showed evidence of regulation by this mechanism. This study is the first to identify undermethylated adenines and cytosines genomewide in a bacterium using second-generation sequencing technology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.