ArticleJournal of bacteriology2013
PelA deacetylase activity is required for Pel polysaccharide synthesis in Pseudomonas aeruginosa.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed, 125 citations in OpenAlex.
- Bacillus cereus PelAThe Journal of biological chemistry · 2026Article
- The Effects of Baicalin in Combination with Cefotaxime on the Biofilm and Metabolic Reprogramming of Multidrug-ResistantBiomolecules · 2026Article
- Advances in bacterial synthesis pathways of bioflocculants and their applications in mine wastewater treatment.World journal of microbiology & biotechnology · 2026Review
- Isolation of Allocrenothrix methanica reveals distinct ecophysiologies of filamentous methanotrophs and adaptations to O2 limitation.The ISME journal · 2026Article
- Genetic Determinants Associated With the Biofilm Formation Impairment in Pseudomonas aeruginosa Clinical Isolates.MicrobiologyOpen · 2025Article
- Identification of Three New Rugose Small Colony Variants from aMicroorganisms · 2025Article
- Role of gene PA2798 on biofilm formation and virulence factors production of Pseudomonas aeruginosa.Folia microbiologica · 2025Article
- Activation of theInfection and immunity · 2025Article
- Biofilm and Outer Membrane Vesicle Formation in ESKAPE Gram-Negative Bacteria: A Comprehensive Review.International journal of molecular sciences · 2025Review
- Extracellular matrix chemistry tunes bacterial biofilm metabolism and optimizes fitness.bioRxiv : the preprint server for biology · 2025Article
- Auto-aggregation inmBio · 2025Article
- Article
- VimR is a conserved master transcription regulator controlling virulence and metabolism in Pseudomonas.Nucleic acids research · 2025Article
- Structural and functional analysis of Pseudomonas aeruginosa PelA provides insight into the modification of the Pel exopolysaccharide.The Journal of biological chemistry · 2025Article
- Structures of α-galactosaminidases from the CAZy GH114 family and homologs defining a new GH191 family of glycosidases.Acta crystallographica. Section D, Structural biology · 2025Article
- Marine-Derived Cytosine Arabinoside (Ara-C) Inhibits Biofilm Formation by Inhibiting PEL Operon Proteins (Pel A and Pel B) of Pseudomonas aeruginosa: An In Silico Approach.Molecular biotechnology · 2025Article
- Does Every Strain ofPathogens (Basel, Switzerland) · 2024Article
- The role of exopolysaccharides Psl and Pel in resistance ofMicrobiology spectrum · 2024Article
- Cultivation in long-term simulated microgravity is detrimental to pyocyanin production and subsequent biofilm formation ability ofMicrobiology spectrum · 2024Article
- The Anti-Microbial Peptide Citrocin ControlsInternational journal of molecular sciences · 2024Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The Pel polysaccharide serves as an intercellular adhesin for the formation and maintenance of biofilms in the opportunistic pathogen Pseudomonas aeruginosa. Pel biosynthesis requires the products of a seven-gene operon, pelA-pelG, all of which are necessary for Pel-dependent biofilm formation and Pel-related phenotypes. One of the genes, pelA, encodes a protein with a predicted polysaccharide deacetylase domain. In this work, the role of the putative deacetylase domain in Pel production was examined. We first established that purified recombinant PelA hydrolyzed the pseudosubstrate p-nitrophenyl acetate in vitro, and site-specific mutations of predicted deacetylase active-site residues reduced activity greater than 10-fold. Additionally, these mutants were deficient in Pel-dependent biofilm formation and wrinkly colony morphology in vivo. Subcellular fractionation experiments demonstrate that PelA localizes to both the membrane and periplasmic fractions. Finally, antiserum against the Pel polysaccharide was generated, and PelA deacetylase mutants do not produce Pel-reactive material. Taken together, these results suggest that the deacetylase activity of PelA is important for the production of the Pel polysaccharide.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.