ArticleSpringerPlus2013
In vitro characterization of a chitosan skin regenerating template as a scaffold for cells cultivation.
Article in SpringerPlus, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The trial behind it
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Who cites it
15 citing papers in PubMed.
- In vitro and in vivo assessment of a non-animal sourced chitosan scaffold loaded with xeno-free umbilical cord mesenchymal stromal cells cultured under macromolecular crowding conditions.Biomaterials and biosystems · 2024Article
- Preparation of laser microporous porcine acellular dermal matrix and observation of wound transplantation.Cell and tissue banking · 2023Article
- Enhanced efficiency in isolation and expansion of hAMSCs via dual enzyme digestion and micro-carrier.Cell & bioscience · 2020Article
- Article
- Biomaterials for Skin Substitutes.Advanced healthcare materials · 2018Review
- Development of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing.Scientific reports · 2018Article
- Progress in development of bioderived materials for dermal wound healing.Regenerative biomaterials · 2017Review
- Review
- Hybrid Nanostructures Containing Sulfadiazine Modified Chitosan as Antimicrobial Drug Carriers.Nanomaterials (Basel, Switzerland) · 2016Article
- Methodologies in creating skin substitutes.Cellular and molecular life sciences : CMLS · 2016Review
- A Bilayer Engineered Skin Substitute for Wound Repair in an Irradiation-Impeded Healing Model on Rat.Advances in wound care · 2015Article
- Vital roles of stem cells and biomaterials in skin tissue engineering.World journal of stem cells · 2015Review
- A simple material model to generate epidermal and dermal layers in vitro for skin regeneration.Journal of materials chemistry. B · 2014Article
- Tissue engineering is a promising method for the repair of spinal cord injuries (Review).Experimental and therapeutic medicine · 2014Article
- Chitosan dermal substitute and chitosan skin substitute contribute to accelerated full-thickness wound healing in irradiated rats.BioMed research international · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chitosan is a marine-derived product that has been widely used in clinical applications, especially in skin reconstruction. The mammalian scaffolds derived from bovine and porcine material have many limitations, for example, prion transmission and religious concerns. Therefore, we created a chitosan skin regenerating template (SRT) and investigated the behavior of fibroblast cell-scaffold constructs. Primary human dermal fibroblasts (HDF) were isolated and then characterized using vimentin and versican. HDF were seeded into chitosan SRT at a density of 3×10(6) cells/cm(2) for fourteen days. Histological analysis and live cells imaging revealed that the cell-chitosan constructs within interconnected porous chitosan showed significant interaction between the cells as well as between the cells and the chitosan. Scanning electron microscopy (SEM) analysis revealed cells spreading and covering the pores. As the pore sizes of the chitosan SRT range between 40-140 μm, an average porosity is about 93 ± 12.57% and water uptake ratio of chitosan SRT is 536.02 ± 14.29%, it is a supportive template for fibroblast attachment and has potential in applications as a dermal substitute.
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