Observational studyPediatric nephrology (Berlin, Germany)2013
Remission of nephrotic syndrome diminishes urinary plasmin content and abolishes activation of ENaC.
Observational study in Pediatric nephrology (Berlin, Germany), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- Urinary plasminogen as an early marker of diabetic kidney disease in children with type 1 diabetes mellitus: a cross-sectional study.European journal of pediatrics · 2025Article
- Urinary Plasminogen as a Marker of Disease Progression in Human Glomerular Disease.American journal of kidney diseases : the official journal of the National Kidney Foundation · 2024Article
- Epithelial NaComprehensive Physiology · 2024Review
- A Serine Protease Inhibitor, Camostat Mesilate, Suppresses Urinary Plasmin Activity and Alleviates Hypertension and Podocyte Injury in Dahl Salt-Sensitive Rats.International journal of molecular sciences · 2023Article
- Regulating ENaC's gate.American journal of physiology. Cell physiology · 2020Review
- The epithelial NaPflugers Archiv : European journal of physiology · 2019Article
- New insights regarding epithelial Na+ channel regulation and its role in the kidney, immune system and vasculature.Current opinion in nephrology and hypertension · 2019Review
- Hypertension in Childhood Nephrotic Syndrome.Frontiers in pediatrics · 2019Review
- Diagnostic and Management Challenges in Congenital Nephrotic Syndrome.Pediatric health, medicine and therapeutics · 2019Review
- Urinary Plasmin(ogen) as a Prognostic Factor for Hypertension.Kidney international reports · 2018Article
- Epithelial NaAnnual review of physiology · 2018Review
- Nephrotic Syndrome: Oedema Formation and Its Treatment With Diuretics.Frontiers in physiology · 2018Review
- Physiology and pathophysiology of the plasminogen system in the kidney.Pflugers Archiv : European journal of physiology · 2017Review
- Acid-base disturbances in nephrotic syndrome: analysis using the COClinical and experimental nephrology · 2017Article
- Podocyte injury: the role of proteinuria, urinary plasminogen, and oxidative stress.American journal of physiology. Renal physiology · 2016Article
- Amiloride and the diabetic kidney.Journal of hypertension · 2016Article
- Role of tubulointerstitial plasmin in the progression of IgA nephropathy.Journal of nephrology · 2016Article
- Sodium retention and volume expansion in nephrotic syndrome: implications for hypertension.Advances in chronic kidney disease · 2015Review
- Urinary serine proteases and activation of ENaC in kidney--implications for physiological renal salt handling and hypertensive disorders with albuminuria.Pflugers Archiv : European journal of physiology · 2015Review
- The epithelial sodium channel γ-subunit is processed proteolytically in human kidney.Journal of the American Society of Nephrology : JASN · 2015Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundUrinary plasmin activates the epithelial Na(+) channel (ENaC) in vitro and may possibly be a mechanism of sodium retention in nephrotic syndrome (NS). This study used a paired design to test the hypothesis that remission of NS is associated with a decreased content of urinary plasmin and reduced ability of patients' urine to activate ENaC.
methodsSamples were collected during active NS and at stable remission from 20 patients with idiopathic NS, aged 9.1 ± 3.2 years. Plasminogen-plasmin concentration was measured with an enzyme-linked immunosorbent assay. Western immunoblotting for plasminogen-plasmin was performed in paired urine samples. The patch clamp technique was used to test the ability of urine to evoke an inward current on collecting duct cells and human lymphocytes.
resultsThe urinary plasminogen-plasmin/creatinine ratio was 226 [95 % confidence interval (CI) 130-503] μg/mmol in nephrotic urine versus 9.5 (95 % CI 8-12) μg/mmol at remission (p < 0.001). Western immunoblotting confirmed the presence of active plasmin in urine collected during active NS, while samples collected at remission were negative. Nephrotic urine generated an inward amiloride- and α2-anti-plasmin- sensitive current, whereas the observed increase in current in urine collected at remission was significantly lower (201 ± 31 vs. 29 ± 10 %; p = 0.005).
conclusionsThese findings support the hypothesis that aberrantly filtered plasminogen-plasmin may contribute to ENaC activation and mediate primary renal sodium retention during active childhood NS.
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