ArticlePflugers Archiv : European journal of physiology2013
Change in Nox4 expression is accompanied by changes in myogenic marker expression in differentiating C2C12 myoblasts.
Article in Pflugers Archiv : European journal of physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 21 citations in OpenAlex.
- Mitochondrial Oxidative Stress and Mitophagy Activation Contribute to TNF-Dependent Impairment of Myogenesis.Antioxidants (Basel, Switzerland) · 2023Article
- Mitochondrial Function and Reactive Oxygen/Nitrogen Species in Skeletal Muscle.Frontiers in cell and developmental biology · 2022Review
- Regulation of skeletal myogenesis in C2C12 cells through modulation of Pax7, MyoD, and myogenin via different low-frequency electromagnetic field energies.Technology and health care : official journal of the European Society for Engineering and Medicine · 2022Article
- NADPH Oxidases: Redox Regulators of Stem Cell Fate and Function.Antioxidants (Basel, Switzerland) · 2021Review
- Mitochondrial Function in Muscle Stem Cell Fates.Frontiers in cell and developmental biology · 2020Review
- NADPH Oxidase 4 Contributes to Myoblast Fusion and Skeletal Muscle Regeneration.Oxidative medicine and cellular longevity · 2019Article
- NADPH Oxidases: Insights into Selected Functions and Mechanisms of Action in Cancer and Stem Cells.Oxidative medicine and cellular longevity · 2017Review
- Skeletal Muscle Regeneration and Oxidative Stress Are Altered in Chronic Kidney Disease.PloS one · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Myoblast differentiation is mediated by a cascade of changes in gene expression including transcription factors such as myogenin. Subsequent to myoblast differentiation, there is an increase in expression of the transmembrane protein NADPH oxidase (Nox). Nox is one of the primary factors for the generation of reactive oxygen species (ROS) in myogenic (C2C12) cells. Recently, ROS have been shown to be important regulators of several intracellular signaling pathways, and the full extent of their regulatory roles is yet to be discovered. In the present study, qRT PCR analysis demonstrated that Nox4 isoform is primarily expressed in differentiating C2C12 cells and contributes to the generation of ROS in C2C12 myoblast during differentiation. Over-expression and silencing of Nox4 expression during myoblast differentiation was accompanied by a reduction in intracellular ROS concentrations and an alteration in the expression patterns of Myf5, Pax7, MyoD1, and myogenin. This modulation was found to be associated with ERK1/2 phosphorylation. In both over-expression and reduced expression of Nox4, we found significant reductions in ERK1/2 phosphorylation. This indicates that cellular differentiation may be affected by Nox4-mediated endogenous ROS generation. These data suggest a new opportunity to study the temporal expression of Nox4 in the generation of ROS accompanying changes in myogenic differentiation.
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