ArticleThe Plant cell2013
Expression of 9-cis-EPOXYCAROTENOID DIOXYGENASE4 is essential for thermoinhibition of lettuce seed germination but not for seed development or stress tolerance.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 115 citations in OpenAlex.
- ABI4-HDA9 Module Epigenetically Silences GIG1 to Accumulate Methylglyoxal for Seed Thermoinhibition Response in Arabidopsis thaliana.Plant, cell & environment · 2026Article
- Functional Analysis of the Heat-Tolerance Candidate GenePlants (Basel, Switzerland) · 2026Article
- Molecular mechanism of abscisic acid regulating bud-break through the NnTIFY10A/B-NnABI5 module in lotus.Horticulture research · 2026Article
- The initiation of embryo growth in imbibed celery mericarps marks a key mechanism by which temperature signals are integrated to regulate germination timing.Journal of experimental botany · 2026Article
- Major Quantitative Trait Loci Control Low-Temperature Germination in Lettuce.Life (Basel, Switzerland) · 2026Article
- Genome-wide transcriptional and metabolic responses of Eschscholzia californica to salt stress.BMC plant biology · 2026Article
- Combined enhancement of ascorbic acid, β-carotene and zeaxanthin in gene-edited lettuce.Plant biotechnology journal · 2025Article
- Mechanism of thermodormancy induction in Fraxinus mandshurica seeds is related to changes in the endosperm.Planta · 2025Article
- Utilizing CRISPR-based genetic modification for precise control of seed dormancy: progress, obstacles, and potential directions.Molecular biology reports · 2025Review
- Genome-wide association studies in lettuce reveal the interplay of seed age, color, and germination under high temperatures.Scientific reports · 2025Article
- A Comprehensive Analysis of the 9-Cis Epoxy Carotenoid Dioxygenase Gene Family and Their Responses to Salt Stress inPlants (Basel, Switzerland) · 2024Article
- Advance in the Thermoinhibition of Lettuce (Plants (Basel, Switzerland) · 2024Review
- Chemical disruption of ABA signaling overcomes high-temperature inhibition of seed germination and enhances seed priming responses.PloS one · 2024Article
- Phosphoproteome analyses pinpoint the F-box protein SLOW MOTION as a regulator of warm temperature-mediated hypocotyl growth in Arabidopsis.The New phytologist · 2024Article
- Scattered differentiation of unlinked loci across the genome underlines ecological divergence of the selfing grassProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Novel flavin-containing monooxygenase protein FMO1 interacts with CAT2 to negatively regulate drought tolerance through ROS homeostasis and ABA signaling pathway in tomato.Horticulture research · 2023Article
- The Arabidopsis endosperm is a temperature-sensing tissue that implements seed thermoinhibition through phyB.Nature communications · 2023Article
- Growth and transcriptional response of wheat and rice to the tertiary amine BMVE.Frontiers in plant science · 2023Article
- Phytochrome B enhances seed germination tolerance to high temperature by reducing S-nitrosylation of HFR1.EMBO reports · 2022Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thermoinhibition, or failure of seeds to germinate at warm temperatures, is common in lettuce (Lactuca sativa) cultivars. Using a recombinant inbred line population developed from a lettuce cultivar (Salinas) and thermotolerant Lactuca serriola accession UC96US23 (UC), we previously mapped a quantitative trait locus associated with thermoinhibition of germination to a genomic region containing a gene encoding a key regulated enzyme in abscisic acid (ABA) biosynthesis, 9-cis-EPOXYCAROTENOID DIOXYGENASE4 (NCED4). NCED4 from either Salinas or UC complements seeds of the Arabidopsis thaliana nced6-1 nced9-1 double mutant by restoring germination thermosensitivity, indicating that both NCED4 genes encode functional proteins. Transgenic expression of Salinas NCED4 in UC seeds resulted in thermoinhibition, whereas silencing of NCED4 in Salinas seeds led to loss of thermoinhibition. Mutations in NCED4 also alleviated thermoinhibition. NCED4 expression was elevated during late seed development but was not required for seed maturation. Heat but not water stress elevated NCED4 expression in leaves, while NCED2 and NCED3 exhibited the opposite responses. Silencing of NCED4 altered the expression of genes involved in ABA, gibberellin, and ethylene biosynthesis and signaling pathways. Together, these data demonstrate that NCED4 expression is required for thermoinhibition of lettuce seeds and that it may play additional roles in plant responses to elevated temperature.
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