ArticleGenetics2013
Novel proteins required for meiotic silencing by unpaired DNA and siRNA generation in Neurospora crassa.
Article in Genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- An Hsp70 Chaperone Is Involved in Meiotic Silencing by Unpaired DNA.Epigenomes · 2026Article
- ARS2, a Cofactor of CBC, Promotes Meiotic Silencing by Unpaired DNA.Epigenomes · 2026Article
- Epigenetic modulation of fungal pathogens: a focus onFrontiers in microbiology · 2024Review
- The Identification and Comparative Analysis of Non-Coding RNAs in Spores and Mycelia ofJournal of fungi (Basel, Switzerland) · 2023Article
- A DEAD-box RNA helicase mediates meiotic silencing by unpaired DNA.G3 (Bethesda, Md.) · 2023Article
- Involvement of RNA granule proteins in meiotic silencing by unpaired DNA.G3 (Bethesda, Md.) · 2021Article
- An NCBP3-Domain Protein Mediates Meiotic Silencing by Unpaired DNA.G3 (Bethesda, Md.) · 2020Article
- TheG3 (Bethesda, Md.) · 2019Article
- Suppressors of Meiotic Silencing by Unpaired DNA.Non-coding RNA · 2019Article
- An RNA Recognition Motif-Containing Protein Functions in Meiotic Silencing by Unpaired DNA.G3 (Bethesda, Md.) · 2017Article
- Ascus dysgenesis in hybrid crosses of Neurospora and Sordaria (Sordariaceae).Journal of genetics · 2017Review
- The Nuclear Cap-Binding Complex Mediates Meiotic Silencing by Unpaired DNA.G3 (Bethesda, Md.) · 2017Article
- A-to-I RNA editing independent of ADARs in filamentous fungi.RNA biology · 2016Article
- Endogenous Small RNA Mediates Meiotic Silencing of a Novel DNA Transposon.G3 (Bethesda, Md.) · 2015Article
- Efficient detection of unpaired DNA requires a member of the rad54-like family of homologous recombination proteins.Genetics · 2014Article
- Recognizing the enemy within: licensing RNA-guided genome defense.Trends in biochemical sciences · 2014Review
- Global gene expression and focused knockout analysis reveals genes associated with fungal fruiting body development in Neurospora crassa.Eukaryotic cell · 2014Article
- RNAi function, diversity, and loss in the fungal kingdom.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2013Review
- Functional diversity of RNAi-associated sRNAs in fungi.International journal of molecular sciences · 2013Review
- Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
During meiosis in the filamentous fungus Neurospora crassa, unpaired genes are identified and silenced by a process known as meiotic silencing by unpaired DNA (MSUD). Previous work has uncovered six proteins required for MSUD, all of which are also essential for meiotic progression. Additionally, they all localize in the perinuclear region, suggesting that it is a center of MSUD activity. Nevertheless, at least a subset of MSUD proteins must be present inside the nucleus, as unpaired DNA recognition undoubtedly takes place there. In this study, we identified and characterized two new proteins required for MSUD, namely SAD-4 and SAD-5. Both are previously uncharacterized proteins specific to Ascomycetes, with SAD-4 having a range that spans several fungal classes and SAD-5 seemingly restricted to a single order. Both genes appear to be predominantly expressed in the sexual phase, as molecular study combined with analysis of publicly available mRNA-seq datasets failed to detect significant expression of them in the vegetative tissue. SAD-4, like all known MSUD proteins, localizes in the perinuclear region of the meiotic cell. SAD-5, on the other hand, is found in the nucleus (as the first of its kind). Both proteins are unique compared to previously identified MSUD proteins in that neither is required for sexual sporulation. This homozygous-fertile phenotype uncouples MSUD from sexual development and allows us to demonstrate that both SAD-4 and SAD-5 are important for the production of masiRNAs, which are the small RNA molecules associated with meiotic silencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.