ArticleJournal of immunological methods2013
Highly multiplexed quantitation of gene expression on single cells.
Article in Journal of immunological methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed.
- Functional, Antigen-Specific Stem Cell Memory (TFrontiers in immunology · 2018Trial
- Antigen Availability Shapes T Cell Differentiation and Function during Tuberculosis.Cell host & microbe · 2017Trial
- Longitudinal dynamics of the HIV-specific B cell response during intermittent treatment of primary HIV infection.PloS one · 2017Trial
- Bacillus Calmette-Guérin (BCG) Revaccination of Adults with Latent Mycobacterium tuberculosis Infection Induces Long-Lived BCG-Reactive NK Cell Responses.Journal of immunology (Baltimore, Md. : 1950) · 2016Trial
- Article
- Single-Cell Profiling of Latently SIV-Infected CD4Microbiology spectrum · 2022Article
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- Prospective multicentre head-to-head validation of host blood transcriptomic biomarkers for pulmonary tuberculosis by real-time PCR.Communications medicine · 2022Article
- Cerebrospinal fluid CD4+ T cell infection in humans and macaques during acute HIV-1 and SHIV infection.PLoS pathogens · 2021Article
- Lipopolysaccharide and tumor necrosis factor-alpha alter gene expression of oocytes and cumulus cells during bovine in vitro maturation.Molecular reproduction and development · 2019Article
- Terminal Effector CD8 T Cells Defined by an IKZF2Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- A deadly dance: the choreography of host-pathogen interactions, as revealed by single-cell technologies.Nature communications · 2018Review
- Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques.Journal of visualized experiments : JoVE · 2018Article
- Elderly human hematopoietic progenitor cells express cellular senescence markers and are more susceptible to pyroptosis.JCI insight · 2018Article
- Altered immune cell follicular dynamics in HIV infection following influenza vaccination.The Journal of clinical investigation · 2018Article
- Rapid HIV RNA rebound after antiretroviral treatment interruption in persons durably suppressed in Fiebig I acute HIV infection.Nature medicine · 2018Article
- Accumulation of follicular CD8+ T cells in pathogenic SIV infection.The Journal of clinical investigation · 2018Article
- Transcriptomic signatures of NK cells suggest impaired responsiveness in HIV-1 infection and increased activity post-vaccination.Nature communications · 2018Article
- Practical considerations for plant phylogenomics.Applications in plant sciences · 2018Review
- Single-cell gene expression of the bovine blastocyst.Reproduction (Cambridge, England) · 2017Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Highly multiplexed, single-cell technologies reveal important heterogeneity within cell populations. Recently, technologies to simultaneously measure expression of 96 (or more) genes from a single cell have been developed for immunologic monitoring. Here, we report a rigorous, optimized, quantitative methodology for using this technology. Specifically: we describe a unique primer/probe qualification method necessary for quantitative results; we show that primers do not compete in highly multiplexed amplifications; we define the limit of detection for this assay as a single mRNA transcript; and, we show that the technical reproducibility of the system is very high. We illustrate two disparate applications of the platform: a "bulk" approach that measures expression patterns from 100 cells at a time in high throughput to define gene signatures, and a single-cell approach to define the coordinate expression patterns of multiple genes and reveal unique subsets of cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.