ArticleJournal of chromatography. B, Analytical technologies in the biomedical and life sciences2013
Evaluating the reproducibility of quantifying modified nucleosides from ribonucleic acids by LC-UV-MS.
Article in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Methylated guanosine and uridine modifications inRSC chemical biology · 2023Article
- Broad-range RNA modification analysis of complex biological samples using rapid C18-UPLC-MS.RNA biology · 2021Article
- Review
- Recent developments in the characterization of nucleic acids by liquid chromatography, capillary electrophoresis, ion mobility, and mass spectrometry (2010-2020).Journal of separation science · 2021Review
- A molecular-level perspective on the frequency, distribution, and consequences of messenger RNA modifications.Wiley interdisciplinary reviews. RNA · 2020Review
- Phase Separation and Disorder-to-Order Transition of Human Brain Expressed X-Linked 3 (hBEX3) in the Presence of Small Fragments of tRNA.Journal of molecular biology · 2020Article
- Identification and Quantification of Modified Nucleosides inACS chemical biology · 2019Article
- Oligonucleotide analysis by hydrophilic interaction liquid chromatography-mass spectrometry in the absence of ion-pair reagents.Journal of chromatography. A · 2019Article
- Assembly and functionality of the ribosome with tethered subunits.Nature communications · 2019Article
- Nano LC-MS using capillary columns enables accurate quantification of modified ribonucleosides at low femtomol levels.RNA (New York, N.Y.) · 2018Article
- Differentiating Positional Isomers of Nucleoside Modifications by Higher-Energy Collisional Dissociation Mass Spectrometry (HCD MS).Journal of the American Society for Mass Spectrometry · 2018Article
- The Effects of Ultraviolet Radiation on Nucleoside Modifications in RNA.ACS chemical biology · 2018Article
- tRNA Modification Detection Using Graphene Nanopores: A Simulation Study.Biomolecules · 2017Article
- Random pseuoduridylation in vivo reveals critical region of Escherichia coli 23S rRNA for ribosome assembly.Nucleic acids research · 2017Article
- Mapping Post-Transcriptional Modifications onto Transfer Ribonucleic Acid Sequences by Liquid Chromatography Tandem Mass Spectrometry.Biomolecules · 2017Review
- Pseudouridine in the Anticodon of Escherichia coli tRNATyr(QΨA) Is Catalyzed by the Dual Specificity Enzyme RluF.The Journal of biological chemistry · 2016Article
- Sequence mapping of transfer RNA chemical modifications by liquid chromatography tandem mass spectrometry.Methods (San Diego, Calif.) · 2016Article
- Attomole quantification and global profile of RNA modifications: Epitranscriptome of human neural stem cells.Nucleic acids research · 2016Article
- Essentiality of threonylcarbamoyladenosine (t(6)A), a universal tRNA modification, in bacteria.Molecular microbiology · 2015Article
- Bidirectional Direct Sequencing of Noncanonical RNA by Two-Dimensional Analysis of Mass Chromatograms.Journal of the American Chemical Society · 2015Article
Corrections and comments
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2 authors.
Funding
Abstract
Post-transcriptional chemical covalent modification of adenosine, guanosine, uridine and cytidine occurs frequently in all types of ribonucleic acids (RNAs). In ribosomal RNA (rRNA) and transfer RNA (tRNA) these modifications make important contributions to RNA structure and stability and to the accuracy and efficiency of protein translation. The functional dynamics, synergistic nature and regulatory roles of these posttranscriptional nucleoside modifications within the cell are not well characterized. These modifications are present at very low levels and isolation of individual nucleosides for analysis requires a complex multi-step approach. The focus of this study is to characterize the reproducibility of a liquid chromatography method used to isolate and quantitatively characterize modified nucleosides in tRNA and rRNA when nucleoside detection is performed using ultraviolet and mass spectrometric detection (UV and MS, respectively). Despite the analytical challenges of sample isolation and dynamic range, quantitative profiling of modified nucleosides obtained from bacterial tRNAs and rRNAs is feasible at relative standard deviations of 5% RSD or less.
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