ArticleDevelopmental biology2013
The ventral to dorsal BMP activity gradient in the early zebrafish embryo is determined by graded expression of BMP ligands.
Article in Developmental biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 69 citations in OpenAlex.
- Real-time single-molecule imaging in zebrafish embryos uncovers noncanonical translation.Science advances · 2026Article
- Biochemical principles of SMAD signaling across the animal kingdom.The Biochemical journal · 2026Review
- Chronic Thermal Stress During Early Zebrafish (Danio rerio) Development Induces Morphological, Molecular, and Liver Histopathological Changes.Fish physiology and biochemistry · 2026Article
- Low-level tetrabromobisphenol A (TBBPA) exposure disrupts early embryonic architecture: molecular impacts on dorsoventral patterning.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · 2026Article
- Disrupted developmental signaling induces novel transcriptional states.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Temporal dynamics of BMP/Nodal ratio drive tissue-specific gastrulation morphogenesis.Development (Cambridge, England) · 2025Article
- Digital cousins: Simultaneous optimization of one model for BMP signaling in distant relatives reveals essential core.Computational and structural biotechnology journal · 2025Article
- Optimal performance objectives in the highly conserved bone morphogenetic protein signaling pathway.NPJ systems biology and applications · 2024Article
- Highly conserved and extremely evolvable: BMP signalling in secondary axis patterning of Cnidaria and Bilateria.Development genes and evolution · 2024Review
- Robust axis elongation by Nodal-dependent restriction of BMP signaling.Development (Cambridge, England) · 2024Article
- An improved Erk biosensor detects oscillatory Erk dynamics driven by mitotic erasure during early development.Developmental cell · 2023Article
- Context-dependent TGFβ family signalling in cell fate regulation.Nature reviews. Molecular cell biology · 2023Review
- Reconstitution of morphogen shuttling circuits.Science advances · 2023Article
- Modeling Human Muscular Dystrophies in Zebrafish: Mutant Lines, Transgenic Fluorescent Biosensors, and Phenotyping Assays.International journal of molecular sciences · 2023Review
- Review
- Patterning principles of morphogen gradients.Open biology · 2022Review
- Differential bioactivity of four BMP-family members as function of biomaterial stiffness.Biomaterials · 2022Article
- Smad4 controls signaling robustness and morphogenesis by differentially contributing to the Nodal and BMP pathways.Nature communications · 2021Article
- Transgenic fluorescent zebrafish lines that have revolutionized biomedical research.Laboratory animal research · 2021Review
- Diversity and robustness of bone morphogenetic protein pattern formation.Development (Cambridge, England) · 2021Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In the early zebrafish embryo, a ventral to dorsal gradient of bone morphogenetic protein (BMP) activity is established, which is essential for the specification of cell fates along this axis. To visualise and mechanistically determine how this BMP activity gradient forms, we have used a transgenic zebrafish line that expresses monomeric red fluorescent protein (mRFP) under the control of well-characterised BMP responsive elements. We demonstrate that mRFP expression in this line faithfully reports BMP and GDF signalling at both early and late stages of development. Taking advantage of the unstable nature of mRFP transcripts, we use in situ hybridisation to reveal the dynamic spatio-temporal pattern of BMP activity and establish the timing and sequence of events that lead to the formation of the BMP activity gradient. We show that the BMP transcriptional activity gradient is established between 30% and 40% epiboly stages and that it is preceded by graded mRNA expression of the BMP ligands. Both Dharma and FGF signalling contribute to graded bmp transcription during these early stages and it is subsequently maintained through autocrine BMP signalling. We show that BMP2B protein is also expressed in a gradient as early as blastula stages, but do not find any evidence of diffusion of this BMP to generate the BMP transcriptional activity gradient. Thus, in contrast to diffusion/transport-based models of BMP gradient formation in Drosophila, our results indicate that the establishment of the BMP activity gradient in early zebrafish embryos is determined by graded expression of the BMP ligands.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.