ArticleDevelopmental cell2013
Chromosome engineering allows the efficient isolation of vertebrate neocentromeres.
Article in Developmental cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 111 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
111 citing papers in PubMed, 167 citations in OpenAlex.
- Dual Roads of CENP-A for Centromere Identification.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Germline-restricted chromosome of songbirds has different centromere compared to regular chromosomes.Heredity · 2026Article
- Augmented CENH3 loading is accompanied by transcriptional and epigenetic reprogramming at rice centromeres during meiosis.Genome biology · 2026Article
- Heterochromatin boundaries maintain centromere position, size and number.Nature structural & molecular biology · 2026Article
- Organization principles of dynamic three-dimensional genome architecture associated with centromere clustering states.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Increased maize chromosome number by engineered chromosome fission.Science advances · 2025Article
- Gross Chromosomal Rearrangement at Centromeres.Biomolecules · 2023Review
- Pan-centromere reveals widespread centromere repositioning of soybean genomes.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Direct recruitment of Mis18 to interphase spindle pole bodies promotes CENP-A chromatin assembly.Current biology : CB · 2023Article
- Flexible Attachment and Detachment of Centromeres and Telomeres to and from Chromosomes.Biomolecules · 2023Review
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- Prospectively defined patterns of APOBEC3A mutagenesis are prevalent in human cancers.Cell reports · 2022Article
- Epigenetic control of centromere: what can we learn from neocentromere?Genes & genomics · 2022Review
- Centromere Identity and the Regulation of Chromosome Segregation.Frontiers in cell and developmental biology · 2022Review
- CENP-A Regulation and Cancer.Frontiers in cell and developmental biology · 2022Review
- Expression of Two Rye CENH3 Variants and Their Loading into Centromeres.Plants (Basel, Switzerland) · 2021Article
- Recent insights into mechanisms preventing ectopic centromere formation.Open biology · 2021Review
- Emerging Single-Cell Technological Approaches to Investigate Chromatin Dynamics and Centromere Regulation in Human Health and Disease.International journal of molecular sciences · 2021Review
- Epigenetic dynamics of centromeres and neocentromeres in Cryptococcus deuterogattii.PLoS genetics · 2021Article
51 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 5 institutions in 2 countries.
Funding
Abstract
Centromeres are specified by sequence-independent epigenetic mechanisms in most organisms. Rarely, centromere repositioning results in neocentromere formation at ectopic sites. However, the mechanisms governing how and where neocentromeres form are unknown. Here, we established a chromosome-engineering system in chicken DT40 cells that allowed us to efficiently isolate neocentromere-containing chromosomes. Neocentromeres appear to be structurally and functionally equivalent to native centromeres. Chromatin immunoprecipitation sequencing (ChIP-seq) analysis with 18 neocentromeres revealed that the centromere-specific histone H3 variant CENP-A occupies an ∼40 kb region at each neocentromere, which has no preference for specific DNA sequence motifs. Furthermore, we found that neocentromeres were not associated with histone modifications H3K9me3, H3K4me2, and H3K36me3 or with early replication timing. Importantly, low but significant levels of CENP-A are detected around endogenous centromeres, which are capable of seeding neocentromere assembly if the centromere core is removed. In summary, our experimental system provides valuable insights for understanding how neocentromeres form.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.