ArticleBMC research notes2013
Validation of reference genes for expression analysis by quantitative real-time PCR in Leptinotarsa decemlineata (Say).
Article in BMC research notes, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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62 citing papers in PubMed.
- Selection and evaluation of reference genes for RT-qPCR normalization across developmental stages and tissues in Dermestes maculatus (Coleoptera: Dermestidae).Journal of insect science (Online) · 2026Article
- Expression and RNAi characterization of neuropeptides underlying developmental regulation in the Colorado potato beetle.Frontiers in insect science · 2026Article
- Bulk Segregant Analysis Reveals Genomic Regions Associated With Imidacloprid Resistance in the Colorado Potato Beetle.Ecology and evolution · 2025Article
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- Control of two insect pests by expression of a mismatch corrected double-stranded RNA in plants.Plant biotechnology journal · 2024Article
- RNA Interference-Mediated Suppression of Ecdysone Signaling Inhibits Choriogenesis in Two Coleoptera Species.International journal of molecular sciences · 2024Article
- Nearly complete genome sequences of the first two identified Colorado potato beetle viruses.Scientific reports · 2024Article
- Evaluation of reference genes for quantitative expression analysis inFrontiers in physiology · 2024Article
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- Identification of sex-specific markers based on gene expression in adult Colorado potato beetles.Journal of insect science (Online) · 2023Article
- Gut microbiota influences feeding behavior via changes in olfactory receptor gene expression in Colorado potato beetles.Frontiers in microbiology · 2023Article
- Expression of Immunity- and Stress-Related Genes during an Intermolt Period in the Colorado Potato Beetle.Insects · 2022Article
- Efficient control of western flower thrips by plastid-mediated RNA interference.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Reference Genes for Expression Analyses by qRT-PCR inInsects · 2022Article
- Identification and Validation of Reference Genes for Gene Expression Analysis inFrontiers in physiology · 2022Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundL. decemlineata is an exotic invasive insect pest, and invaded in Xinjiang Uygur autonomous region in China in the 1990s from Kazakhstan. It is a notorious defoliator of potato throughout most of the northern Xinjiang in current, and often causes extremely large yield losses of potato.
resultsThe expression stability of nine L. decemlineata house-keeping genes (Actin, ACT1 and ACT2; ADP-ribosylation factor, ARF1 and ARF4; TATA box binding protein, TBP1 and TBP2; ribosomal protein RP4 and RP18; translation elongation factor 1α EF1α) was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR) in seven developmental stages, three larval tissues and two insecticide treatments. The results were analyzed using three software programs: geNorm, NormFinder and BestKeeper. Although there was no consistent ranking observed among the house-keeping genes across the samples, the overall analysis revealed that RP18, RP4, ARF1, and ARF4 were the four most stable house-keeping genes. In contrast, ACT1 and ACT2, two of the most widely used reference genes, had the least stability. Our results suggest that the combined use of the four most stably expressed genes may produce optimal normalization for qRT-PCR.
conclusionsThe expression stability of the house-keeping genes varies among different developing stages, in different tissues and under different experimental conditions. Our results will enable a more accurate and reliable normalization of qRT-PCR data in L. decemlineata.
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