ArticleBMC research notes2013
Construction of a high-quality yeast two-hybrid (Y2H) library and its application in identification of interacting proteins with key vernalization regulator TaVRN-A1 in wheat.
Article in BMC research notes, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Deciphering cassava brown streak virus infection in cassava through VPg mediated host protein interactions.Archives of virology · 2026Article
- Construction of yeast two-hybrid cDNA library and identification of interacting protein with McHDRa/b in Matricaria chamomilla L.Plant molecular biology · 2025Article
- The E3 ligase TaE3V-B1 ubiquitinates proteins encoded by the vernalization gene TaVRN1 and regulates developmental processes in wheat.Plant physiology · 2024Article
- Genome‑wide analysis of the GT8 gene family in apple and functional identification of MhGolS2 in saline-alkali tolerance.Plant molecular biology · 2024Article
- The TaSOC1-TaVRN1 module integrates photoperiod and vernalization signals to regulate wheat flowering.Plant biotechnology journal · 2024Article
- Overexpression ofActa biochimica et biophysica Sinica · 2023Article
- Screening and characterisation of proteins interacting with the mitogen-activated protein kinase Crmapk in the fungus Clonostachys chloroleuca.Scientific reports · 2022Article
- O-linked N-acetylglucosamine transferase is involved in fine regulation of flowering time in winter wheat.Nature communications · 2021Article
- Yeast two-hybrid screening for proteins that interact with PFT in wheat.Scientific reports · 2019Article
- Characterization ofThe plant pathology journal · 2019Article
- GhCyP3 improves the resistance of cotton to Verticillium dahliae by inhibiting the E3 ubiquitin ligase activity of GhPUB17.Plant molecular biology · 2019Article
- Construction of a kiwifruit yeast two-hybrid cDNA library to identify host targets of the Pseudomonas syringae pv. actinidiae effector AvrPto5.BMC research notes · 2019Article
- Augmentation of crop productivity through interventions of omics technologies in India: challenges and opportunities.3 Biotech · 2018Review
- The STENOFOLIA gene from Medicago alters leaf width, flowering time and chlorophyll content in transgenic wheat.Plant biotechnology journal · 2018Article
- TaXA21-A1 on chromosome 5AL is associated with resistance to multiple pests in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Article
- Vernalization requirement duration in winter wheat is controlled by TaVRN-A1 at the protein level.The Plant journal : for cell and molecular biology · 2013Article
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Abstract
backgroundLow temperature is required for the competence of winter wheat to flowering (vernalization), and several key components in the vernalization-mediated flowering pathway have been isolated. A Y2H library is a very useful platform to further unravel novel regulators in the flowering pathway. Thus, there is a necessity to construct a high-quality Y2H library using vernalized winter wheat plants.
resultWe described the construction of a high-quality Y2H library using winter wheat plants with cold-treatment for different weeks to maximize pooling interacting proteins during vernalization. The resultant Y2H library contained ~2.5×10(6) independent clones, with a cell density of ~2.6×10(8) and an average insert size of ~ 1.5 kb. TaVRN-A1 was used as a "bait" to test the quality of the Y2H library. As a result, several cDNA clones encoding TaSOC1 and TaSVP1 that were known to have a direct binding with TaVRN-A1 were identified, demonstrating that the Y2H screen system constructed in this study was highly efficient. Additional proteins that were discovered but not characterized in previous studies could be novel partners of TaVRN-A1 in wheat.
conclusionWe established a high-efficient Y2H screen system using the Matchmaker™ technology with several modifications in the critical steps. Ultimately, we provided a successful example to fast and economically create high-quality Y2H libraries for studies on protein interaction in hexaploid wheat.
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