ArticleBMC genomics2013
Characterization and differential expression of microRNAs in the ovaries of pregnant and non-pregnant goats (Capra hircus).
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Gene regulation by non-Coding RNAs in infertility: a mechanistic review.Journal of ovarian research · 2025Review
- Transcriptome analysis reveals miRNA expression profiles in hypothalamus tissues during the sexual development of Jining grey goats.BMC genomics · 2024Article
- Dynamic Expression of Follicle-Stimulating Hormone and Estrogen mRNA Receptors Associated with microRNAsAnimals : an open access journal from MDPI · 2024Article
- Expression profiles of circulating miRNAs in an endangered Piedmontese sheep breed during the estrus cycle.Frontiers in veterinary science · 2024Article
- Characterization of MicroRNA expression profiles in the ovarian tissue of goats during the sexual maturity period.Journal of ovarian research · 2023Article
- Integrative Analysis of miRNA-mRNA in Ovarian Granulosa Cells Treated with Kisspeptin in Tan Sheep.Animals : an open access journal from MDPI · 2022Article
- miRNA expression patterns in blood leukocytes and milk somatic cells of goats infected with small ruminant lentivirus (SRLV).Scientific reports · 2022Article
- Review
- miRNA Regulatory Functions in Farm Animal Diseases, and Biomarker Potentials for Effective Therapies.International journal of molecular sciences · 2021Review
- Genome-wide transcriptome profiling uncovers differential miRNAs and lncRNAs in ovaries of Hu sheep at different developmental stages.Scientific reports · 2021Article
- Expression Profile Analysis to Identify Circular RNA Expression Signatures in the Prolificacy Trait of Yunshang Black Goat Pituitary in the Estrus Cycle.Frontiers in genetics · 2021Article
- Extracellular microRNA profiling in human follicular fluid: new biomarkers in female reproductive potential.Journal of assisted reproduction and genetics · 2020Review
- Analysis of pituitary transcriptomics indicates that lncRNAs are involved in the regulation of sheep estrus.Functional & integrative genomics · 2020Article
- Comparative expression analysis of let-7 microRNAs during ovary development in Megalobrama amblycephala.Fish physiology and biochemistry · 2019Article
- Article
- High-throughput sequencing of small RNAs and analysis of differentially expressed microRNAs associated with high-fat diet-induced hepatic insulin resistance in mice.Genes & nutrition · 2019Article
- Distribution of ncRNAs expression across hypothalamic-pituitary-gonadal axis in Capra hircus.BMC genomics · 2018Article
- Identification and characterization of microRNAs in the pituitary of pubescent goats.Reproductive biology and endocrinology : RB&E · 2018Article
- Identification and comparative analysis of the ovarian microRNAs of prolific and non-prolific goats during the follicular phase using high-throughput sequencing.Scientific reports · 2017Article
- Placental microRNA expression in pregnancies complicated by superimposed pre‑eclampsia on chronic hypertension.Molecular medicine reports · 2016Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOvarian follicular development and hormone secretion are complex and coordinated biological processes which will usually be altered during pregnancy. Ovarian function is tightly regulated by a multitude of genes, and also by some specific miRNAs. It is necessary to identify the differentially expressed miRNAs in the ovaries of pregnant and non-pregnant mammals, in order to further understand the role of miRNA-mediated post-transcriptional regulation in mammalian reproduction. Here, we performed a comprehensive search for hircine miRNAs using two small RNA sequencing libraries prepared from the ovaries of pregnant and non-pregnant goats.
results617 conserved and 7 putative novel miRNAs were identified in the hircine ovaries. A total of 471 conserved miRNAs (76.34%) were co-expressed in both pregnant and non-pregnant libraries, and 90 pregnancy-specific and 56 non-pregnancy-specific conserved miRNAs were identified. Additionally, 407 unique miRNAs (65.96%) were significantly differentially expressed in the pregnant and non-pregnant libraries, of which 294 were upregulated and 113 were downregulated in the pregnant library compared to the non-pregnant library. Further analysis showed that miR-143 was predicted to bind to the target sequences of Frizzled-6 and -3 receptor genes in the Wnt/beta-catenin signaling pathway, and let-7b may target the Activin receptor I and Smad 2/3 genes in the TGF-beta signaling pathway. The expression level of 5 randomly selected miRNAs were analyzed by quantitative real-time PCR (q-PCR), and the results demonstrated that the expression patterns were consistent with the Solexa sequencing results.
conclusionsThe identification and characterization of differentially expressed miRNAs in the ovaries of pregnant and non-pregnant goats provides important information on the role of miRNA in the regulation of the ovarian development and function. This data will be helpful to facilitate studies on the regulation of miRNAs during mammalian reproduction.
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