ArticleBMC genomics2013
Global proteomic analysis of the oocyst/sporozoite of Toxoplasma gondii reveals commitment to a host-independent lifestyle.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 84 citations in OpenAlex.
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- Genome-wide transcriptomic analysis of Toxoplasma gondii reveals stage-specific regulatory programs and metabolic adaptations driving oocyst sporulation.BMC genomics · 2026Article
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- Proteomics Applications inPathogens (Basel, Switzerland) · 2023Review
- Comparative proteomic analysis of wall-forming bodies and oocyst wall reveals the molecular basis underlying oocyst wall formation in Eimeria necatrix.Parasites & vectors · 2023Article
- Unravelling the sexual developmental biology ofFrontiers in cellular and infection microbiology · 2023Article
- Enrichment and proteomic identification of Cryptosporidium parvum oocyst wall.Parasites & vectors · 2022Article
- The transcriptome from asexual to sexual in vitro development of Cystoisospora suis (Apicomplexa: Coccidia).Scientific reports · 2022Article
- The flexibility of Apicomplexa parasites in lipid metabolism.PLoS pathogens · 2022Review
- Comparative Phosphoproteomic Analysis of Sporulated Oocysts and Tachyzoites ofMolecules (Basel, Switzerland) · 2022Article
- New Avenues to DesignFrontiers in immunology · 2022Article
- Identification of Oocyst-DrivenMicroorganisms · 2021Review
- Sexual Development in Non-Human Parasitic Apicomplexa: Just Biology or Targets for Control?Animals : an open access journal from MDPI · 2021Review
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- Characterization of Cystoisospora suis sexual stages in vitro.Parasites & vectors · 2020Article
- Reduction of Allergic Lung Disease by Mucosal Application ofFrontiers in immunology · 2020Article
- Structure, composition, and roles of theCell surface (Amsterdam, Netherlands) · 2019Review
- Environmental transmission ofFood and waterborne parasitology · 2019Review
- Functional Characterization and Structure-Guided Mutational Analysis of the Transsulfuration Enzyme Cystathionine γ-Lyase fromInternational journal of molecular sciences · 2018Article
- Novel proteome and acetylome of Bemisia tabaci Q in response to Cardinium infection.BMC genomics · 2018Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundToxoplasmosis is caused by the apicomplexan parasite Toxoplasma gondii and can be acquired either congenitally or via the oral route. In the latter case, transmission is mediated by two distinct invasive stages, i.e., bradyzoites residing in tissue cysts or sporozoites contained in environmentally resistant oocysts shed by felids in their feces. The oocyst plays a central epidemiological role, yet this stage has been scarcely investigated at the molecular level and the knowledge of its expressed proteome is very limited.
resultsUsing one-dimensional gel electrophoresis coupled to liquid chromatography-linked tandem mass spectrometry, we analysed total or fractionated protein extracts of partially sporulated T. gondii oocysts, producing a dataset of 1304 non reduntant proteins (~18% of the total predicted proteome), ~59% of which were classified according to the MIPS functional catalogue database. Notably, the comparison of the oocyst dataset with the extensively covered proteome of T. gondii tachyzoite, the invasive stage responsible for the clinical signs of toxoplasmosis, identified 154 putative oocyst/sporozoite-specific proteins, some of which were validated by Western blot. The analysis of this protein subset showed that, compared to tachyzoites, oocysts have a greater capability of de novo amino acid biosynthesis and are well equipped to fuel the Krebs cycle with the acetyl-CoA generated through fatty acid β-oxidation and the degradation of branched amino acids.
conclusionsThe study reported herein significantly expanded our knowledge of the proteome expressed by the oocyst/sporozoite of T. gondii, shedding light on a stage-specifc subset of proteins whose functional profile is consistent with the adaptation of T. gondii oocysts to the nutrient-poor and stressing extracellular environment.
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