ArticleDevelopment genes and evolution2013
RNA interference in the appendicularian Oikopleura dioica reveals the function of the Brachyury gene.
Article in Development genes and evolution, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 33 citations in OpenAlex.
- Formation of the brain by stem cell divisions of large neuroblasts in Oikopleura dioica, a simple chordate.Development genes and evolution · 2023Article
- 3D reconstruction of structures of hatched larva and young juvenile of the larvacean Oikopleura dioica using SBF-SEM.Scientific reports · 2021Article
- Massive Gene Loss and Function Shuffling in Appendicularians Stretch the Boundaries of Chordate Wnt Family Evolution.Frontiers in cell and developmental biology · 2021Article
- A chordate species lackingProceedings of the National Academy of Sciences of the United States of America · 2020Article
- ANISEED 2017: extending the integrated ascidian database to the exploration and evolutionary comparison of genome-scale datasets.Nucleic acids research · 2018Article
- Modified whole-mount in situ hybridisation and immunohistochemistry protocols without removal of the vitelline membrane in the appendicularian Oikopleura dioica.Development genes and evolution · 2017Article
- DNA interference-mediated screening of maternal factors in the chordate Oikopleura dioica.Scientific reports · 2017Article
- Maternal and zygotic transcriptomes in the appendicularian, Oikopleura dioica: novel protein-encoding genes, intra-species sequence variations, and trans-spliced RNA leader.Development genes and evolution · 2015Article
- Tunicates: exploring the sea shores and roaming the open ocean. A tribute to Thomas Huxley.Open biology · 2015Review
- DNA interference: DNA-induced gene silencing in the appendicularian Oikopleura dioica.Proceedings. Biological sciences · 2015Article
- Functional specialization of chordate CDK1 paralogs during oogenic meiosis.Cell cycle (Georgetown, Tex.) · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The appendicularian Oikopleura dioica is a chordate that has a remarkably simple adult body with small cell number. Its transparency, stereotyped cell lineages, short life cycle, and small genome make it a promising new experimental model of chordate developmental biology. However, the functions of its various genes are still poorly understood due to lack of a tool for suppression of gene expression. Here, we applied a double-stranded RNA (dsRNA)-based-RNA interference (RNAi) method in O. dioica. For introducing dsRNA into eggs and embryos, we injected dsRNAs into the ovary. dsRNA, which is specific to EGFP or mCherry mRNA, decreased the exogenous mRNA-derived fluorescence in both eggs and embryos. dsRNA specific to the Brachyury gene of O. dioica, which is a homologous gene of a key notochord transcriptional factor in ascidians, triggered degradation of endogenous Brachyury mRNA and induced malformation or loss of the notochord in the tail. This effect was Brachyury sequence specific, as three dsRNAs covering different sequences produced the same phenotype. The result is in accordance with its expression site and also with the key regulatory function of Brachyury in notochord formation in other chordates. RNAi in O. dioica would be a useful tool for gaining insight into the oogenesis and early developmental processes in chordates.
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