ArticleIranian journal of basic medical sciences2012
Identification of Nontuberculous Mycobacteria Species Isolated from Water Samples Using Phenotypic and Molecular Methods and Determination of their Antibiotic Resistance Patterns by E- Test Method, in Isfahan, Iran.
Article in Iranian journal of basic medical sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Trial
- Comparative evaluation of MALDI-ToF mass spectrometry and Sanger sequencing of theFrontiers in cellular and infection microbiology · 2025Article
- Isolation and identification of non-tuberculous mycobacteria from aquarium fish in Ilam, Iran.Journal of clinical tuberculosis and other mycobacterial diseases · 2024Article
- Isolation of nontuberculous mycobacteria species from different water sources: a study of six hospitals in Tehran, Iran.BMC microbiology · 2022Article
- The source and fate of Mycobacterium tuberculosis complex in wastewater and possible routes of transmission.BMC public health · 2022Article
- Heat-killedFood science & nutrition · 2021Article
- Review
- Safety of heat-killedEFSA journal. European Food Safety Authority · 2019Article
- Statins improve survival in patients previously treated with nivolumab for advanced non-small cell lung cancer: An observational study.Molecular and clinical oncology · 2019Article
- Article
- Prevalence of Non-Tuberculous Mycobacteria in Hospital Waters of Major Cities of Khuzestan Province, Iran.Frontiers in cellular and infection microbiology · 2016Article
- Article
- Molecular characterization of Aspergillus infections in an Iranian educational hospital using RAPD-PCR method.Iranian journal of basic medical sciences · 2014Article
- Citation Analysis of Iranian Journal of Basic Medical Sciences in ISI Web of Knowledge, Scopus, and Google Scholar.Iranian journal of basic medical sciences · 2013Article
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4 authors.
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Abstract
introductionMany studies have shown epidemiological links between strains isolated in tap water, and those isolated from patients. Molecular methods linked to PCR are more reliable and faster for identification of non- tuberculous mycobacteria (NTM). In this study molecular methods were used for identification and typing of NTM. MATERIALS AND
methodsFive hundred ml of 85 water samples was passed through 0.45 μm filters. The filters were transferred directly onto 7H10 Middle Brook solid media, containing 15% OADC. PCR for 16S rRNA was done and the PCR product (1500 bp) was sequenced. PRA of the hsp65 gene was investigated to identify the species of isolates. For evaluation of susceptibility of NTM to antimycobacterial agents, E-test method was used.
resultThe genus of 26 isolated NTM was confirmed by 16s rRNA sequence based method. Nineteen isolates of Mycobacteria were differentiated using hsp65 genes PRA. The dominant isolates were M. fortuitum (26.7%), M. chelonae like organism (13.3%) and M. mucogenicum (13.3%). Seventy one percent of NTM species were resistant to isoniazid, 64% to rifampin, 57% to ethambutol, 35% to tetracycline, 14 % to azithromycin and 7.1 % to amikacin.
conclusionThe results showed that E-test method is not a proper technique for antimycobacterial assay because some NTM species are slow in growing and have no growth on Muller Hinton agar. Regarding the 16S rRNA sequence analysis, the identification of isolates was restricted to the genus level, because 99% similarity within 16S rRNA of two isolates may or may not determine the same species.
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