ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2013
Mining the "glycocode"--exploring the spatial distribution of glycans in gastrointestinal mucin using force spectroscopy.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- Determination of Gene-Level Fitness Contributions Across the Mucin-Utilization Network of Bacteroides thetaiotaomicron.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Novel Perspectives on the Relationship Between the Gastrointestinal Mucus Barrier and Soybean Agglutinin.Cells · 2026Review
- Exploring bioadhesion: insight on innovative strategies to investigate bioadhesive scaffolds.International journal of pharmaceutics: X · 2025Review
- Dysbiosis-associated gut bacteriumMicrobiology spectrum · 2025Article
- Article
- Mucin-driven ecological interactions in an in vitro synthetic community of human gut microbes.Glycobiology · 2024Article
- Exploring the sequence-function space of microbial fucosidases.Communications chemistry · 2024Article
- New carbohydrate binding domains identified by phage display based functional metagenomic screens of human gut microbiota.Communications biology · 2023Article
- Article
- Mucosal glycan degradation of the host by the gut microbiota.Glycobiology · 2021Review
- Interaction Between Chitosan and Mucin: Fundamentals and Applications.Biomimetics (Basel, Switzerland) · 2019Review
- Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Getting the feel of food structure with atomic force microscopy.Food hydrocolloids · 2018Article
- Mechanistic Insights Into the Cross-Feeding ofFrontiers in microbiology · 2018Article
- Spatial organization of a model 15-member human gut microbiota established in gnotobiotic mice.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Secondary Structure and Glycosylation of Mucus Glycoproteins by Raman Spectroscopies.Analytical chemistry · 2016Article
- Use of Atomic Force Microscopy to Study the Multi-Modular Interaction of Bacterial Adhesins to Mucins.International journal of molecular sciences · 2016Article
- The mucin-degradation strategy of Ruminococcus gnavus: The importance of intramolecular trans-sialidases.Gut microbes · 2016Article
- Carbohydrate-binding specificities of potential probiotic Lactobacillus strains in porcine jejunal (IPEC-J2) cells and porcine mucin.Journal of microbiology (Seoul, Korea) · 2016Article
- The biogeography of polymicrobial infection.Nature reviews. Microbiology · 2016Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Mucins are the main components of the gastrointestinal mucus layer. Mucin glycosylation is critical to most intermolecular and intercellular interactions. However, due to the highly complex and heterogeneous mucin glycan structures, the encoded biological information remains largely encrypted. Here we have developed a methodology based on force spectroscopy to identify biologically accessible glycoepitopes in purified porcine gastric mucin (pPGM) and purified porcine jejunal mucin (pPJM). The binding specificity of lectins Ricinus communis agglutinin I (RCA), peanut (Arachis hypogaea) agglutinin (PNA), Maackia amurensis lectin II (MALII), and Ulex europaeus agglutinin I (UEA) was utilized in force spectroscopy measurements to quantify the affinity and spatial distribution of their cognate sugars at the molecular scale. Binding energy of 4, 1.6, and 26 aJ was determined on pPGM for RCA, PNA, and UEA. Binding was abolished by competition with free ligands, demonstrating the validity of the affinity data. The distributions of the nearest binding site separations estimated the number of binding sites in a 200-nm mucin segment to be 4 for RCA, PNA, and UEA, and 1.8 for MALII. Binding site separations were affected by partial defucosylation of pPGM. Furthermore, we showed that this new approach can resolve differences between gastric and jejunum mucins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.